Effects of fluctuating glucose levels on neuronal cells in vitro.

Neurochemical Research
Vincenzo C RussoFergus J Cameron

Abstract

There is increasing evidence for glucose fluctuation playing a role in the damaging effects of diabetes on various organs, including the brain. We aimed to study the effects of glycaemic variation (GV) upon mitochondrial activity using an in vitro human neuronal model. The metabolic disturbance of GV in neuronal cells, was mimicked via exposure of neuroblastoma cells SH-SY5Y to constant glucose or fluctuating (i.e. 6 h cycles) for 24 and 48 h. Mitochondrial dehydrogenase activity was determined via MTT assay. Cell mitochondrial activity (MTT) was moderately decreased in constant high glucose, but markedly decreased following 24 and 48 h of cyclical glucose fluctuations. Glucose transport determined via 2-deoxy-D-[1-(14)C] glucose uptake was regulated in an exaggerated manner in response to glucose variance, accompanied by modest changes in GLUT 1 mRNA abundance. Osmotic components of these glucose effects were investigated in the presence of the osmotic-mimics mannitol and L: -glucose. Both treatments showed that fluctuating osmolality did not result in a significant change in mitochondrial activity and had no effects on (14)Cglucose uptake, suggesting that adverse effects on mitochondrial function were specifically related to ...Continue Reading

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Citations

Nov 13, 2014·Journal of Pediatric Endocrinology & Metabolism : JPEM·Ganesh S JevalikarMatthew A Sabin
Jul 27, 2015·Pediatric Clinics of North America·Fergus J Cameron
Jan 26, 2013·Diabetes·Elisabeth A Northam, Fergus J Cameron
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Sep 7, 2017·Neurotoxicity Research·Susana CardosoPaula I Moreira
Nov 16, 2018·Frontiers in Neurology·Young-Kook KimJuhyun Song
Feb 7, 2021·Diabetes Therapy : Research, Treatment and Education of Diabetes and Related Disorders·Xingyao TangRafael Simó
Dec 30, 2020·Nutrients·Charles WattJanice Jin Hwang

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