Effects of fluoroquinolones on CYP4501A and 3A in male broilers

Research in Veterinary Science
Ling-Ling ZhangShan-Xiang Jiang

Abstract

The inhibitory effects of fluoroquinolones on the enzyme activity, protein levels and mRNA expression of liver cytochrome P450 (CYP) 1A and 3A were investigated in male broiler chicks. Enrofloxacin (20 mg/kg), sarafloxacin (8 mg/kg) and marbofloxacin (5.5 mg/kg) were administrated in drinking water for 7 consecutive days. A cocktail of the probe drugs caffeine and dapsone was used to determine CYP1A and 3A activity. Western blot analysis and real-time PCR were used to determine the effects on protein levels of CYP1A and 3A, and on CYP1A4, 1A5, 3A37 mRNA levels. Enrofloxacin increased the half-life of elimination for both caffeine and dapsone, and decreased expression of CYP1A and 3A protein. Marbofloxacin decreased the metabolism of caffeine and expression of CYP1A protein. However, no change in mRNA expression was observed for any treatment group. This suggested that high doses of enrofloxacin and marbofloxacin, but not sarafloxacin, inhibit CYP in chick liver raising the possibility of drug-drug interaction when using these compounds.

References

Feb 1, 1985·Journal of Pharmaceutical Sciences·F C LamD G Perrier
Aug 1, 1995·Journal of Veterinary Pharmacology and Therapeutics·A ShlosbergS Soback
Jan 1, 1996·Canadian Journal of Physiology and Pharmacology·M CouletP Galtier
Jan 6, 2000·Archives of Biochemistry and Biophysics·J C OurlinU A Meyer
Oct 24, 2000·Toxicology and Applied Pharmacology·J M JacobsJ F Sinclair
Nov 7, 2001·Journal of Veterinary Pharmacology and Therapeutics·W F KhalilE Kokue
Oct 22, 2003·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·R A HirtR van den Hoven
Aug 26, 2004·British Journal of Clinical Pharmacology·Ashish SharmaBettina A Hamelin
Dec 14, 2005·Journal of Veterinary Pharmacology and Therapeutics·N L RegmiM Shimoda
May 9, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Akira KubotaShinsuke Tanabe
Jan 16, 2007·Journal of Veterinary Pharmacology and Therapeutics·N L RegmiM Shimoda
Aug 19, 2007·Research in Veterinary Science·Anu RahalJ K Malik
Oct 22, 2008·Nutrition & Metabolism·Zakia Bibi

❮ Previous
Next ❯

Citations

Aug 1, 2013·Indian Journal of Pharmaceutical Sciences·R A XuJ S Ma
Jun 12, 2013·European Journal of Drug Metabolism and Pharmacokinetics·Myung-Jin ChoiSeung-Chun Park
May 28, 2011·Journal of Veterinary Pharmacology and Therapeutics·N LiuS-X Jiang
Jul 6, 2011·Journal of Veterinary Pharmacology and Therapeutics·X HuJ-F Zhou
Aug 2, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Chang ZhouJun-Fang Zhou
Jan 12, 2020·Biopharmaceutics & Drug Disposition·Wei HuangXiao Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.