Effects of follicle-stimulating hormone and ovarian steroids during in vitro meiotic maturation on fertilization of rat oocytes

Gamete Research
X Zhang, D T Armstrong

Abstract

The present study examined the effects of gonadotropins and ovarian steroids during in vitro meiotic maturation of rat oocytes on their ability to undergo in vitro fertilization. Fully grown oocytes were isolated from antral follicles of immature rats and cultured as oocyte-cumulus cell complexes (OCC) under conditions in which completion of meiotic maturation occurs spontaneously. They were then exposed to spermatozoa under conditions in which oocytes matured in vivo exhibit high fertilization rates. Compared with oocytes from pregnant mare's serum gonadotropin (PMSG) or follicle-stimulating hormone (FSH)-treated rats, a similar proportion of the oocytes (greater than 80%) from untreated rats underwent germinal vesicle breakdown, but such oocytes had a lower rate of fertilization (70% vs. 20%). The presence of FSH during in vitro maturation restored the fertilization rate for oocytes from untreated rats, while a cytochrome P450 inhibitor, aminoglutethimide phosphate abolished this beneficial effect of FSH. The addition of progesterone during the in vitro maturation period duplicated the beneficial effect of FSH on fertilization rate of oocytes from untreated rats; oestradiol-17 beta was less effective in this regard, and 5 alp...Continue Reading

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Citations

Feb 1, 1997·Molecular Reproduction and Development·T C WoodD E Wildt
Aug 1, 1990·Molecular Reproduction and Development·B C Vanderhyden, D T Armstrong
Mar 1, 1991·Molecular Reproduction and Development·X ZhangD T Armstrong
May 1, 1992·Molecular Reproduction and Development·X ZhangD T Armstrong
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Feb 7, 2006·Reproductive Biology and Endocrinology : RB&E·Hai Feng WangTakato Terada
Jul 1, 2004·The Journal of Reproduction and Development·Qing LiMorag G Hunter
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Jan 23, 2002·Molecular Reproduction and Development·M E ChiappeP Saragüeta
Mar 28, 2020·Journal of Assisted Reproduction and Genetics·Irene ZolfaroliAntonio Cano

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