Effects of follicular size and FSH on granulosa cell apoptosis and atresia in porcine antral follicles

Molecular Reproduction and Development
Pengfei Lin, Rong Rui

Abstract

The purpose of this study was to establish a culture model for isolated intact porcine antral follicles and investigate the relationship between granulosa cell apoptosis and follicular atresia. Small (<3 mm), medium (3-5 mm) and large (>5 mm) healthy porcine follicles were isolated and cultured in serum-free TCM199 with or without follicular stimulating hormone (FSH). Microscopic identification of healthy follicles was confirmed by histology. A spontaneous onset of apoptotic cell death in granulosa cells was observed from cultured antral follicles. The apoptotic rate of granulosa cells from small follicles cultured for 24 hr was higher than those of large and medium follicles, accompanied with high FasL mRNA abundance in granulosa cells. Supplementation with 3 or 5 IU/ml FSH significantly inhibited the percentage of granulosa cells that became apoptotic. FSH did not significantly alter estradiol secretion from cultured follicles. Progesterone secretion significantly decreased after culture for 48 hr, coinciding with the morphological changes observed. FasL and Fas mRNA were expressed in the healthy, early atretic, and progressed atretic porcine follicles regardless of follicular size. However, FasL but not Fas mRNA levels incre...Continue Reading

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Feb 21, 2013·Journal of Assisted Reproduction and Genetics·Marina C PeluffoMary B Zelinski
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Jun 1, 2021·Molecular Human Reproduction·Tomoya NakanishiYasuhisa Yamashita

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis