Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores

FEMS Microbiology Letters
Marcelo Barraza-SalasMario Pedraza-Reyes

Abstract

The effects of overexpression of the apurinic/apyrimidinic DNA endonuclease Nfo on wet and dry heat and UV-C (254 nm) resistance of Bacillus subtilis spores with or without alpha/beta-type small, acid-soluble spore proteins (SASP) were determined. Results revealed that overexpression of Nfo > or =50-fold in spores increased the wet heat resistance of exoA nfo B. subtilis spores (termed alpha(-)beta(-)) that lack most alpha/beta-type SASP, but had no effect on these spores' UV-C resistance. Nfo overexpression also increased these spores' dry heat resistance, and to levels slightly greater than that of wild-type spores. These results are consistent: (1) with wet and dry heat (but not UV-C) generating abasic sites in alpha(-)beta(-) spore DNA; (2) with dry heat generating some of these lesions in spores that retain alpha/beta-type SASP; and (3) indicate that Nfo can repair these abasic lesions following spore germination.

References

Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B Setlow, P Setlow
Jun 1, 1972·Journal of Medical Genetics·K Takano, J R Miller
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·W L NicholsonP Setlow
Apr 3, 2003·Journal of Bacteriology·Rosa-Martha Cabrera-MartinezPeter Setlow
Dec 17, 2003·Journal of Nanobiotechnology·Ezio Ricca, Simon M Cutting
Feb 25, 2006·Journal of Molecular Biology·Stephanie T WangPatrick Eichenberger
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen
Oct 17, 2006·Journal of Applied Microbiology·M PaulP Setlow
Mar 6, 2007·Trends in Microbiology·Peter Setlow
Sep 25, 2007·Journal of Bacteriology·William H ColemanPeter Setlow
May 26, 2009·Trends in Microbiology·Wayne L Nicholson

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Citations

Apr 2, 2013·Trends in Microbiology·Kai ReinekeDietrich Knorr
Jan 7, 2020·Annual Review of Food Science and Technology·Kai Reineke, Alexander Mathys
Jan 1, 2011·Aerosol Science and Technology : the Journal of the American Association for Aerosol Research·Elisabet JohanssonSergey A Grinshpun
Jun 25, 2015·Microbiology Spectrum·Peter Setlow

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