PMID: 18710611Aug 20, 2008Paper

Effects of FRZB on growth and metastasis of gastric cancer cell line SGC-7901

Ai zheng = Aizheng = Chinese journal of cancer
Ying QuZheng-Gang Zhu

Abstract

FRZB (frizzled motif associated with bone development), a member of the secreted frizzled related protein (sFRP) family, plays an important role in embryonic development. The expression of FRZB can suppress the invasion ability of prostate cancer by inhibiting the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. This study was to investigate the effects of FRZB on tumorigenicity and metastasis of gastric cancer cell line SGC-7901. FRZB expression vector and empty vector were constructed and transfected into SGC-7901 cells to obtain stable clones (SGC-7901/FRZB and SGC-7901/vector). Cell proliferation was detected by MTT assay. The tumorigenicity was investigated by soft agar colony formation test (in vitro) and xenograft mouse model (in vivo). The adhesive and invasive abilities of SGC-7901/FRZB cells were determined by Boyden chambers. The expression of MMPs in SGC-7901 cells was tested by immunocytochemistry. The expression of FRZB was increased in SGC-7901/FRZB cells. Growth rate and colony formation rate of SGC-7901/FRZB cells were decreased to about 60% and 15% as compared with those of SGC-7901/vector cells, respectively. The rates of adhesion to collagenI, collagen IV, vitronectin, fibronectin and laminin were...Continue Reading

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