PMID: 2509618Sep 1, 1989Paper

Effects of FSH and testosterone on highly purified rat Sertoli cells: inhibin alpha-subunit mRNA expression and inhibin secretion are enhanced by FSH but not by testosterone

The Journal of Endocrinology
A M ToeboschJ A Grootegoed

Abstract

The effects of FSH and testosterone on inhibin mRNA expression and inhibin production by highly purified Sertoli cell preparations were examined. Sertoli cells were isolated from testes of 22-day-old rats by sequential trypsin, collagenase and hyaluronidase treatments, with subsequent osmotic shock treatment on day 3 of culture. Contamination by peritubular and germ cells was less than 0.5 and 1-3% respectively. Intracellular and secreted inhibin levels were measured by radioimmunoassay, using Sertoli cells which were incubated for 24 h in the absence or presence of FSH and testosterone from days 4 to 5 of culture. FSH stimulated the cellular inhibin content and the secreted inhibin level by four- and sevenfold respectively, with a half-maximal effective dose of 5-50 ng/ml. Under the present incubation conditions, testosterone (1 mumol/l) had no effect on immunoreactive inhibin levels in either the presence or absence of FSH. Similarly, the expression of inhibin alpha-subunit mRNA was increased following FSH stimulation, whereas testosterone had no effect. The expression of inhibin beta B-subunit mRNAs was not influenced by FSH or testosterone. It is concluded that highly purified Sertoli cell preparations, with a very low numb...Continue Reading

Citations

Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·C D Kochakian, A A Welder
Mar 26, 1990·Molecular and Cellular Endocrinology·A J GrootenhuisF H de Jong
Nov 1, 1991·Molecular and Cellular Endocrinology·N GérardB Jégou
May 4, 2002·Molecular and Cellular Endocrinology·Mahmoud Huleihel, Eitan Lunenfeld
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C PineauB Jégou
Apr 26, 2011·The Journal of Biological Chemistry·Anne-Pascale SatieNathalie Dejucq-Rainsford
May 15, 2010·Molecular & Cellular Proteomics : MCP·Marilena D PapaioannouSerge Nef
Nov 22, 2002·Biology of Reproduction·Nathalie MelaineBernard Jégou
Dec 21, 2002·Biology of Reproduction·Emmanuelle ComCharles Pineau
Apr 25, 2000·Biology of Reproduction·D ZeyseM Huleihel
Mar 20, 2002·American Journal of Reproductive Immunology : AJRI·Mahmoud HuleihelMoshe Mazor
Jul 12, 2002·Molecular Reproduction and Development·Catherine CelebiThierry Guillaudeux
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·C D Kochakian, A A Welder
Nov 2, 2005·Reproductive Medicine and Biology·Nobue KanematsuMadoka Suto

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