Effects of FSS on the expression and localization of the core proteins in two Wnt signaling pathways, and their association with ciliogenesis

International Journal of Molecular Medicine
Xin ShengDayu Li

Abstract

Fluid shear stress (FSS) may alter ciliary structures and ciliogenesis, and it has been reported that the Wnt signaling pathway may regulate cilia assembly and disassembly. The present study aimed to investigate the effects of FSS on primary cilia, the Wnt/β‑catenin and Wnt/PCP signaling pathways, and the association among them. In the present study, human umbilical vein endothelial cells were subjected to FSS of differing velocities for various periods of time using a shear stress device. Subsequently, immunofluorescence and quantitative polymerase chain reaction were used to detect the expression and localization of the following core proteins: β‑catenin in the Wnt/β‑catenin signaling pathway; and dishevelled segment polarity protein 2 (Dvl2), fuzzy planar cell polarity protein (Fuz) and VANGL planar cell polarity protein 2 (Vangl2) in the Wnt/planar cell polarity (PCP) signaling pathway. Furthermore, the colocalization of Dvl2 with the basal body was analyzed under low FSS and laminar FSS. The results demonstrated that low FSS promoted the expression of Dvl2 and its colocalization with the basal body. Although Fuz expression was decreased with increasing duration of FSS, no visible alterations were detected in its localizati...Continue Reading

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Citations

Aug 11, 2020·Histochemistry and Cell Biology·Xin ShengJunhua Wang
Dec 21, 2018·Emerging Topics in Life Sciences·Christina M Warboys
Jun 14, 2021·European Biophysics Journal : EBJ·Ilaria TortorellaFrancesco Morena

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Methods Mentioned

BETA
protein extraction
electrophoresis

Software Mentioned

Fuz
Quantity One
SPSS

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