Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption

International Journal of Molecular Sciences
Runhong LeiGengmei Xing

Abstract

The excellent biocompatibility and biological effects of fullerenol and its derivatives make their biomedical application promising. The potential effects of fullerenol in mammals have been extensively studied, but little is known about its effects on female reproduction. Using canonical oocyte-granulosa cell complexes (OGCs) in vitro maturation culture model, we investigated the effect of fullerenol on the first oocyte meiotic resumption. In the surrounding granulosa cells, fullerenol nanoparticles occluded the extracellular domain of the epidermal growth factor receptor (EGFR) to reduce EGFR-ligand binding and subsequent extracellular signal-regulated kinase 1 and 2 (ERK1/2) activation, which involved the regulation of connexin 43 (CX43) expression and internalization. Downregulation of CX43 expression and the retraction of transzonal projections (TZPs) interrupted the gap junction channel and TZPs based mass transportation. This effect decreased cyclic adenosine monophosphate (cAMP) levels in the oocyte and thereby accelerated rat oocyte meiosis resumption. Moreover, perinuclear distribution of CX43 and EGFR was observed in granulosa cells, which could further exacerbate the effects. Fullerenol nanoparticles interfered with ...Continue Reading

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Citations

Jul 23, 2020·Reproduction, Fertility, and Development·Monica De Los ReyesGeorge Ramirez
Mar 11, 2020·Frontiers in Genetics·Mengfen WangXiangdong Liu
Jun 8, 2021·Reproductive Toxicology·Ramsés Santacruz-MárquezIsabel Hernández-Ochoa
Sep 24, 2021·Molecular Reproduction and Development·José Roberto Viana SilvaRicardo Pires Dos Santos

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Methods Mentioned

BETA
confocal microscopy
electrophoresis
fluorescence imaging

Software Mentioned

Prism
ImageJ

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