PMID: 12763093May 24, 2003Paper

Effects of gamma2S subunit incorporation on GABAA receptor macroscopic kinetics

Neuropharmacology
Andrew J BoileauR A Pearce

Abstract

GABA(A) receptors, the major inhibitory neurotransmitter receptors in the mammalian central nervous system, are heteropentameric proteins. We are interested in understanding the contribution of the gamma subunit to the kinetic properties of GABA(A) receptors. Studies in Xenopus oocytes have suggested that co-expression of alpha1, beta2, and gamma 2S subunits results in the formation of both alpha beta and alpha betagamma receptors (Boileau et al. 2002a; Boileau et al., 1998). Here, we have used an excess of the gamma 2S subunit in transfections of HEK293 cells to bias expression toward alpha beta gamma-containing receptors. Using rapid application and whole cell patch clamp techniques, we found that incorporation of the gamma subunit eliminated the rapid phases of desensitization and accelerated deactivation, consistent with a proposed role of desensitization in slowing deactivation. In addition, alpha betagamma receptors had an increased GABA EC(50), reduced sensitivity to block by Zn(2+), and did not display outward rectification as compared to alpha beta receptors.

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Citations

Dec 6, 2006·Pflügers Archiv : European journal of physiology·Srinivasan P VenkatachalanAndrew J Boileau
Mar 2, 2005·Nature Reviews. Neuroscience·Mark Farrant, Zoltan Nusser
Dec 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrew J BoileauCynthia Czajkowski
Apr 28, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark D EyreZoltan Nusser
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ataúlfo Martínez-Torres, Ricardo Miledi
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Frédéric Minier, Erwin Sigel
Sep 1, 2012·Cellular and Molecular Life Sciences : CMLS·Angelo Keramidas, Joseph W Lynch
Jul 1, 2011·The European Journal of Neuroscience·Pamela Lachance-TouchettePatrick Cossette
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Jan 31, 2009·Organic Letters·Jürgen Ramharter, Johann Mulzer
Jun 2, 2021·Cellular and Molecular Life Sciences : CMLS·Erwan SallardPascal Legendre

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