Effects of genistein on angiotensin-converting enzyme in rats

Life Sciences
Yin-Yan XuSheng-Nan Li

Abstract

Genistein (4,5,7-trihydroxyisoflavone), a phytoestrogen with selective estrogen receptor modulator properties, has received a great deal of attention over the last few years because of its potentially preventive roles against cardiovascular diseases. However, the precise molecular mechanisms for this modulation are not fully elucidated. In this study, we investigated (both in vivo and in vitro) the relationship between genistein and the changes of angiotensin-converting enzyme (ACE) in rat aortic endothelial cells (RAECs), serum and tissue (aorta). ACE expression was assessed by the immunofluorescence and the reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Serum and tissue ACE activity was detected with a commercial kit. Genistein exhibited a concentration-dependent inhibitory effect on the expression of ACE, particularly at higher concentrations (24.70+/-1.20 at 100microM, P<0.01, and 18.22+/-0.92 at 200microM, P<0.01 compared with the control group 50.49+/-5.19). The estrogen receptor blocker tamoxifen at 100microM attenuated this effect of genistein. The extracellular signal-regulated kinase 1/2 (ERK1/2) blocker PD98059 also markedly inhibited this effect. The observations in vivo were highly consistent with ...Continue Reading

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Citations

Nov 3, 2012·The British Journal of Nutrition·Nallasamy Palanisamy, Anuradha C Venkataraman
Aug 21, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hsin-Yi YangLe-Shin Chang
Nov 18, 2008·Canadian Journal of Physiology and Pharmacology·Juejin WangShengnan Li
Feb 24, 2009·European Journal of Pharmacology·Marcelo F MontenegroJose E Tanus-Santos
Oct 15, 2013·The Journal of Nutritional Biochemistry·Maria E FrigoletArmando R Tovar
Dec 21, 2017·Nutrients·Anna Rzepecka-StojkoEwa Buszman
Jun 24, 2017·Journal of Complementary & Integrative Medicine·Mohammad Yaseen Khan, Vimal Kumar
Apr 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Rzepecka-StojkoJerzy Stojko
Apr 14, 2015·Jundishapur Journal of Natural Pharmaceutical Products·Nader Pazyar, Reza Yaghoobi
Feb 10, 2021·Antioxidants·Anuson PoasakatePoungrat Pakdeechote
Jul 17, 2009·The Journal of Physical Chemistry. B·Vincenza CrupiValentina Venuti

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