Effects of GH and IGF-I administration on GHRH and somatostatin mRNA levels: I. A study on ad libitum fed and starved adult male rats

Journal of Endocrinological Investigation
M C GhigoE E Müller

Abstract

The individual role played by GH and IGF-I in the regulation of hypothalamic GHRH and SRIF gene expression is still object of debate. We have investigated the effect of exogenously administered recombinant hGH (rhGH) and recombinant hIGF-I (rhIGF-I) in ad libitum fed control and starved rats, the latter an animal model which is characterized by low circulating levels of endogenous GH and IGF-I. Adult male rats were fed ad libitum (C) or food-deprived (S) for 72 hours; rats in either C or S groups were treated with systemic administration of rhGH and rhIGF-I for 3 days. GHRH, SRIF and GH mRNA levels were evaluated by Northern and slot blot hybridization. Administration of rhGH (250 micrograms/kg/twice daily, sc) induced a significant inhibition of GHRH and a significant stimulation of SRIF mRNA levels in C rats; GH treatment was, however, ineffective on both neuropeptide mRNA levels in the S group. Continuous infusion of rhIGF-I (300 micrograms/kg/day, sc) induced a significant increase of SRIF levels in both C and S rats but did not modify GHRH mRNA levels in either group. In the pituitary, GH mRNA levels followed a pattern very similar to that of GHRH. These results provide evidence for a direct role of GH in the inhibition of...Continue Reading

References

Dec 1, 1978·Clinical Endocrinology·M C SheppardB L Pimstone
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·M J WatersW I Wood
Jan 1, 1970·Physiology & Behavior·F B AltafferP D'encarnacao
Nov 1, 1994·Endocrinology·R R Reinhardt, C A Bondy
Feb 1, 1994·Endocrine Reviews·J P ThissenL E Underwood
Oct 1, 1995·Journal of Endocrinological Investigation·G P Ceda
Feb 1, 1997·Neuroendocrinology·R S BroganW B Wehrenberg

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Citations

Mar 25, 2008·Molecular and Cellular Endocrinology·E Burgos-RamosE Arilla-Ferreiro
Feb 9, 2008·Molecular and Cellular Endocrinology·Raul M LuqueRhonda D Kineman
May 3, 2005·General and Comparative Endocrinology·Laura E Nelson, Mark A Sheridan
Jan 13, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anderson O L WongWendy K W Ko
Jun 2, 2005·Domestic Animal Endocrinology·L L AndersonA Glavaski-Joksimovic
Oct 1, 2008·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Eunhee KimSeungjoon Park
Apr 30, 1999·Physiological Reviews·E E MüllerD Cocchi
Nov 15, 2012·Journal of Translational Medicine·Juan E Puche, Inma Castilla-Cortázar

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