PMID: 16613825Apr 15, 2006Paper

Effects of GH and IGF-I on the in vitro maturation of mouse oocytes

Hormones : International Journal of Endocrinology and Metabolism
Erasmia KiapekouAristidis Antsaklis

Abstract

A number of hormones and growth factors have been reported to affect the in vitro maturation of oocytes. Their exact effects on follicular growth and oocyte maturation and the mechanisms involved are still unclear. In the present study, we have investigated the effects of Growth Hormone (GH) and Insulin-like Growth Factor 1 (IGF-1) on the in vitro maturation of mouse oocytes. Immature preovulatory oocytes without cumulus cells (denuded), were obtained from 4- to 8-week old female mice and were cultured in Ham's F-10 medium. GH and IGF-1 were added separately or in combination in gradually increasing concentrations in the culture media, while medium-only containing samples were employed as controls. Oocyte development was assessed daily for three days and maturation was considered to be completed when the first polar body appeared. In control samples, 44+/-3% (mean+/-SE) of denuded oocytes formed a polar body. The achieved maturation rate after the addition of either GH or IGF-1 or GH plus IGF-1 was significantly higher than in controls. The highest maturation rates were achieved after the addition of 0.2 microg/ml GH (76%+/-5%), 50 ng/ml IGF-1 (69%+/-5%) and a combination of 0.2 microg/ml GH plus 10 ng/ml IGF-1 (75%+/-5%). The ...Continue Reading

Citations

Oct 26, 2010·Endocrine·S Harvey
Apr 23, 2009·Reproduction, Fertility, and Development·A K Alhaider, P F Watson
Feb 19, 2008·Journal of Assisted Reproduction and Genetics·Jenna K Nyholt de Prada, Catherine A VandeVoort
Dec 15, 2015·General and Comparative Endocrinology·Rui ZhouWei Ge
Feb 20, 2007·Annals of the New York Academy of Sciences·Dimitris LoutradisAristidis Antsaklis
Feb 20, 2007·Annals of the New York Academy of Sciences·Erasmia KiapekouAristidis Antsaklis
Jan 18, 2011·Fertility and Sterility·Erasmia KiapekouDimitris Loutradis
Jan 13, 2015·International Journal of Endocrinology·Kerry L Hull, Steve Harvey
Oct 25, 2016·Clinical Medicine Insights. Endocrinology and Diabetes·Jesús DevesaPablo Devesa
Apr 4, 2009·Reproductive Biology and Endocrinology : RB&E·Yinghui YeToshinobu Tanaka
Jul 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ye YuanR Michael Roberts
Dec 8, 2017·The Journal of Reproduction and Development·Akane SatoYoshikazu Nagao
Aug 10, 2019·Frontiers in Endocrinology·Yue LiHan Zhao
Mar 12, 2021·Biology of Reproduction·Jianzhen LiChristopher H K Cheng

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