Effects of GH on IGF-II-induced progesterone accumulation by cultured porcine granulosa cells

Endocrine
Y P XuP A Thacker

Abstract

A total of seven experiments were conducted to investigate the potential facilitative interaction of growth hormone (GH) and insulin-like growth factor II (IGF-II) in stimulating steroidogenesis by cultured porcine granulosa cells and to examine the possible nature of this action. Porcine granulosa cells were cultured in serum-free medium in the presence or absence of GH or prolactin, with or without IGF-II or IGF-I. IGF-II by itself dose (with peak progesterone production of 498 ng/mg DNA/24 h being observed at 100 ng of IGF-II/mL) and time- (with minimum time requirement of 24-48 h) dependently increased progesterone accumulation (P < 0.01). Neither GH (dose range 0, 5, 10, 50, 100, and 150 ng/mL) nor prolactin (dose range 0, 0.01, 0.1, 1, 5, 10 micrograms/mL) alone stimulated progesterone accumulation when compared with the control (P > 0.05). However, in the presence of IGF-II, GH proved to be a potent amplifier of IGF-II in progesterone production (P < 0.01) with a minimum GH time requirement of 24-48 h. In contrast, prolactin did not influence IGF-II-induced progesterone accumulation (P > 0.05). An inhibitory study showed that the presence of cycloheximide (3 micrograms/mL) or actinomycin D (1 microgram/mL) blocked both t...Continue Reading

References

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Nov 1, 1990·Trends in Endocrinology and Metabolism : TEM·D R Clemmons

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Citations

May 23, 2000·Life Sciences·N YamashitaN Yamashita
Mar 4, 2000·Animal Reproduction Science·E L GregoraszczukA Gertler
Oct 20, 1999·Domestic Animal Endocrinology·R J ScaramuzziB K Campbell
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S HarveyK L Hull
Sep 26, 2003·Psychosomatic Medicine·Tanja LangeJan Born

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