Effects of ghrelin on homocysteine-induced dysfunction and inflammatory response in rat cardiac microvascular endothelial cells

Cell Biology International
Dongjuan WangFeng Cao

Abstract

Ghrelin is a well-characterized hormone that has protective effects on endothelial cells. Elevated HCY (homocysteine) can be a cardiovascular risk factor, but it is not known whether ghrelin can inhibit HCY-induced dysfunction and inflammatory response in rat CMECs (cardiac microvascular endothelial cells). We found that HCY treatment for 24 h inhibited proliferation and NO (nitric oxide) secretion, but with increased cell apoptosis and secretion of cytokines in CMECs. In contrast, ghrelin pretreatment significantly improved proliferation and NO secretion, and inhibited cell apoptosis and secretion of cytokines in HCY-induced CMECs. In addition, Western blot assay showed that NF-κB (nuclear factor κB) and cleaved-caspase 3 expression were elevated, and PCNA (proliferating cell nuclear antigen) and eNOS (endothelial nitric oxide synthase) expression were decreased after treatment with HCY, which was significantly reversed by pretreatment with ghrelin. The data suggest that ghrelin inhibits HCY-induced CMEC dysfunction and inflammatory response, probably mediated by inhibition of NF-κB activation.

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Citations

May 14, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Eugenia Mercedes LuqueAna Carolina Martini
Feb 11, 2014·Archives of Medical Research·Yanbo ChenChun-hua Zhao
Apr 15, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Flavia Prodam, Nicoletta Filigheddu
Dec 9, 2014·The International Journal of Biochemistry & Cell Biology·Emilie MartinezBlandine Comte
May 8, 2018·Hypertension·Xiuchuan LiYongjian Yang
Jul 20, 2016·International Journal of Molecular Medicine·Bin LiRuijie Xie

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