Abstract
Artificial abnormal microenvironment caused by microperfusion of L-glutamate (Glu) and Ca2+ in the hippocampus results in neuron damage, which is closely related to cerebral ischemia. Ginsenoside Rb1, a compound from Panax notoginseng, was previously used to counter the artificial abnormal hippocampal environment in a microperfusion model. In addition, while the Akt/mTOR/PTEN signaling pathway has been shown to mediate neuronprotection in cerebral ischemia, whether this pathway is involved in the neuroprotection of ginsenoside Rb1 is unknown. Here SH-SY5Y cells exposed to OGD/R injury in treated with LY294002, ginsenoside Rb1, ginsenoside Rb1+ LY294002. Expressions of phosphorylation (P-)Akt/P-mTOR/P-PTEN (24 h after OGD/R) were detected by Western blotting. Effects were examined via the memory function of rats (by Morris water maze test), morphological changes in pyramidal cell (by histology), and mRNA expression (by qRT-PCR) and phosphorylation (P-) (by Western blotting and immunohistochemical staining) of Akt, P-mTOR, and P-PTEN in the hippocampus. The memory deficit of rats and pyramidal cellular necrosis and apoptosis in the CA1 region of hippocampus after microperfusion of Glu and Ca2+ were dose dependently alleviated by ...Continue Reading
References
Jul 1, 1997·Neuroscience Research·J H LimM Sakanaka
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Nov 1, 2007·The Journal of Biological Chemistry·Volker MeskeThomas Georg Ohm
Oct 15, 2010·Journal of Cellular Physiology·Minghui Jessica ChenNam Sang Cheung
Dec 17, 2011·Neuroscience·J ZhuX Liu
Dec 22, 2011·Neurobiology of Disease·Joana ArbeloaCarlos Matute
Feb 14, 2012·Brain Research·Bei PengJun Zhou
Feb 15, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Kyoichi KairaNobuyuki Yamamoto
May 2, 2012·European Journal of Pharmacology·Shu ZhangXie-nan Huang
Sep 1, 2012·Neurochemical Research·Rui-Qin YaoXiu-Xiang Wu
Oct 2, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Ryo HashimotoTetsuro Okabe
Apr 4, 2013·The American Journal of Chinese Medicine·Zhou JiangQuan Xie
Nov 14, 2013·PloS One·Di LiuMengren Zhang
Sep 11, 2014·Neural Regeneration Research·Juan LiuQionglan Yuan
Sep 27, 2014·International Journal of Molecular Sciences·Tianfei LuoJianmin Liang
Mar 1, 2015·Neuroscience·G ZhuJ Wang
May 6, 2015·International Journal of Surgery·Ruipeng SongLimin Wang
Jun 11, 2015·Rejuvenation Research·Kai ShenBoyang Yu
Jun 14, 2015·Experimental Brain Research·Wei ChenWusong Tong
Jul 15, 2015·Brain Research·Li ZhouTeng-Fei Ma
Jan 6, 2016·Cell and Tissue Research·Jun GuoTian-Zhi Zhao
Feb 2, 2016·Neuroscience Letters·Yan-Zhuo ZhangChun-Yu Song
Nov 9, 2016·BioMed Research International·Xiao-Xiao LuQiong Chen
Dec 14, 2016·Molecular Cancer Therapeutics·Yueh-Hsiung KuoFeng-Yao Tang
Jan 10, 2017·Journal of Ethnopharmacology·Shiyun WangLijin Pu
Mar 10, 2017·Platelets·Taryn N GreenMaggie L Kalev-Zylinska
Citations
Dec 19, 2020·Epigenomics·Qijie ZhaoZhangang Xiao
Dec 29, 2020·Neuroscience Letters·Ying GuoRongping Zhang
Sep 6, 2020·Journal of Ethnopharmacology·Huamin WeiJing Yu
Jan 28, 2022·Phytotherapy Research : PTR·Jing LuXiaofang Li