Effects of glucocorticoids and interleukin-1 beta on expression and activity of aggrecanases in equine chondrocytes

American Journal of Veterinary Research
Evita BusschersDean W Richardson

Abstract

To determine effects of interleukin (IL)-1 beta and glucocorticoids on total glycosaminoglycan (GAG) loss and aggrecanase-mediated matrix degradation in equine cartilage. Cartilage from 24 equine cadavers free of sepsis and musculoskeletal disease. Effects of IL-1 beta, IL-1 beta with glucocorticoids (dexamethasone and triamcinolone, 10(-6) and 10(-7)M), and glucocorticoids alone on degradation of equine articular and nasal cartilage explants were assessed by measuring GAG release in media and GAG content in cartilage. Aggrecanase-mediated cleavage within the interglobular domain at Glu373-Ala374 was evaluated via western blot analysis and ELISAs. Steady-state mRNA concentrations of matrix metalloproteinase (MMP)-3, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)4, and ADAMTS5 were assessed by use of real-time reverse transcriptase PCR assay (cartilage explants) and northern blot analysis (cell culture). IL-1 beta increased GAG release and aggrecanase activity (11-fold). The MMP-3, MMP-13, and ADAMTS4 mRNA were upregulated with IL-1 beta, whereas ADAMTS5 mRNA was increased (13-fold), but significantly less than ADAMTS4 mRNA (27-fold), suggesting a role for both ADAMTS4 and ADAMTS5 in degradation ...Continue Reading

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Citations

Mar 21, 2018·BMC Veterinary Research·Mariana Castro MartinsLuis M Rubio-Martinez
Aug 21, 2013·Journal of Cellular and Molecular Medicine·Jiadi WenPeter C K Leung
Aug 28, 2020·Frontiers in Veterinary Science·Stasia N SullivanSushmitha S Durgam
Jan 3, 2021·Research in Veterinary Science·Alessandra Di SalvoGiorgia Della Rocca

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