Effects of glucocorticoids on lipid metabolism and AMPK in broiler chickens' liver

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Xiyi HuZhigang Song

Abstract

Adenosine monophosphate-activated protein kinase (AMPK) plays a pivotal role in the regulation of carbohydrate, lipid, and protein metabolism in animals. In this study, we examined whether any cross talk exists between glucocorticoids and AMPK in the regulation of the liver bile acid biosynthesis pathway. Dexamethasone treatment decreased the growth performance of broiler chickens. The liver mRNA levels of fatty acid transport protein (FATP-1), farnesoid X receptor (FXR), AMPK alpha 1 subunit (AMPKα1), and glucocorticoid receptor were significantly upregulated in DEX-treated broilers; the gene expression of liver cholesterol 7 alpha-hydroxylase (CYP7A1) was significantly downregulated. The protein level of liver CYP7A1 was significantly decreased by DEX treatment at both 24 and 72 h, while the protein level of p-AMPK/ t-AMPK stayed unchanged. In the in vitro cultured hepatocytes, compound C pretreatment blocked the increase in CYP7A1 protein level by DEX and significantly suppressed FATP-1, SREBP-1c, FXR, and CYP7A1 gene expression stimulated by DEX. Compound C treatment significantly reduces the protein level of p-AMPK, and the combination of compound C and DEX significantly reduces the protein level of t-AMPK. Thus, glucocort...Continue Reading

Citations

Jan 14, 2021·International Journal of Molecular Sciences·Carine BeaupereGhislaine Guillemain
May 16, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Xiyi HuZhigang Song
Jan 1, 2022·Animal Biotechnology·Wenxin LiWeimin Wang

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