PMID: 16532834Mar 15, 2006Paper

Effects of glucose and free fatty acids on apoptosis of human vascular endothelial cells

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Jin SuHaoming Tian

Abstract

This study sought to assess effect of high level of glucose or free fatty acids (FFAs) on apoptosis of vascular endothelial cell, and to examine whether the combined presence of high concentrations of glucose and FFAs may cross-amplify their individual detrimental effect. Cultured endothelial cell sline (ECV304) were incubated with various concentrations of glucose and/or selected FFAs (palmitate and/or oleate) for 24-96 h. Microscopic changes were observed by electron microscopy. DNA fragmentation was visualized by agarose gel electrophoresis. Apoptotic percentage of endothelial cells were determined using flow cytometry. After treatment with 30 mmol/L of glucose, 0.25 of palmitate (PA) and 0.5 mmol/L of oleate (OA) respectively, typical apoptosis morphological changes including condensed chromatin, nuclear fragmentation, reduction in volume and apoptotic bodies were observed under electron microscope, and the agarose gel electrophoresis of DNA from cells treated with high levels of glucose and FFAs revealed "Ladder" pattern. Both high concentrations of glucose and FFAs elicited apoptosis in a time- and dose-dependent manner in endothelial cells, and flow cytometry showed the cells were mainly blocked at G0/G1 phase. The apopt...Continue Reading

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis