PMID: 44196Dec 15, 1979

Effects of glucose on the cytosolic ration of reduced/oxidized nicotinamide-adenine dinucleotide phosphate in rat islets of Langerhans

The Biochemical Journal
S J Ashcroft, M R Christie

Abstract

The maximal extractable activity of "malic" enzyme (EC 1.1.1.40) in rat islets of Langerhans was similar to that reported for liver. Thus "malic" enzyme may catalyse a near-equilibrium reaction in the cytosol of islets of Langerhans. Measurements of islet content of malate and pyruvate, the metabolite substrate and product of "malic" enzyme, were therefore used to calculate the cytosolic ration of [NADPH]/[NADP+]. This ratio was higher in islets incubated with 20 mM-glucose than in islets incubated with 2 mM-glucose.

References

Aug 28, 2002·Diabetes·Leonard A Fahien, Michael J MacDonald
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Patrick E MacDonaldPatrik Rorsman
Oct 15, 1984·Experientia·W J MalaisseA Sener
Jul 1, 1985·Cell Biochemistry and Function·H P Ammon, M Mark
Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, Franz M Matschinsky
Nov 15, 2006·The Journal of Biological Chemistry·Rebecca L PongratzGary W Cline
Sep 2, 2016·Physiological Reviews·David G Nicholls
May 12, 1998·The American Journal of Physiology·G Magnus, J Keizer
Oct 15, 2005·The Journal of Biological Chemistry·Huasheng LuAjay Verma
Oct 9, 2020·Signal Transduction and Targeted Therapy·Na XieBingwen Zou

Related Concepts

Malate Dehydrogenase
Pyruvate Measurement
Glucose, (beta-D)-Isomer
Pyruvate
NADP
Malate
Oxidation-Reduction
Metabolite
Malates
Islets of Langerhans

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