PMID: 6798217Nov 1, 1981Paper

Effects of glutaraldehyde or osmium tetroxide fixation on the osmotic properties of lung cells

Journal of Microscopy
D WangensteenE R Weibel

Abstract

The osmotic properties of lung cells have been tested before and after perfusion fixation of isolated, perfused lungs with either glutaraldehyde or osmium tetroxide. The testing procedure was to add hypertonic sucrose to the perfusate for several minutes and monitor the lung weight response (an 'osmotic transient'). Each lung was perfused with one or the other fixative solutions for 10 min, then the perfusate was changed back to Ringer-lactate before the post-fixation test was conducted. The results indicate that osmium tetroxide makes the cell membranes as permeable to sucrose as to water, and that sucrose thus causes no osmotic volume change. Glutaraldehyde, on the other hand, apparently preserves the impermeability of the cell membranes to sucrose, but the osmotic volume response is attenuated, indicating that significant changes in the cells have occurred.

References

Nov 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J GilE R Weibel
Jul 1, 1977·Microvascular Research·O D WangensteenP Savaryn
Jun 1, 1971·The Journal of Cell Biology·Q Bone, E J Denton
Nov 1, 1967·Journal of Theoretical Biology·J A Johnson, T A Wilson
Dec 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·D WangensteenE R Weibel

Citations

Feb 5, 2010·American Journal of Respiratory and Critical Care Medicine·Connie C W HsiaATS/ERS Joint Task Force on Quantitative Assessment of Lung Structure
Jul 29, 2006·Journal of Microscopy·M Ochs
Jan 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jan Philipp Schneider, M Ochs
Sep 7, 2019·Acta Neuropathologica Communications·Whitney C McFaddenAndrew T McKenzie
Jan 1, 1984·The American Journal of Physiology·J Gil, J M McNiff

Related Concepts

Aldehydes
Fixatives
Sekumatic
Lung
Osmium
Osmium Tetroxide
Water-Electrolyte Balance

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.