PMID: 6798217Nov 1, 1981Paper

Effects of glutaraldehyde or osmium tetroxide fixation on the osmotic properties of lung cells

Journal of Microscopy
D WangensteenE R Weibel


The osmotic properties of lung cells have been tested before and after perfusion fixation of isolated, perfused lungs with either glutaraldehyde or osmium tetroxide. The testing procedure was to add hypertonic sucrose to the perfusate for several minutes and monitor the lung weight response (an 'osmotic transient'). Each lung was perfused with one or the other fixative solutions for 10 min, then the perfusate was changed back to Ringer-lactate before the post-fixation test was conducted. The results indicate that osmium tetroxide makes the cell membranes as permeable to sucrose as to water, and that sucrose thus causes no osmotic volume change. Glutaraldehyde, on the other hand, apparently preserves the impermeability of the cell membranes to sucrose, but the osmotic volume response is attenuated, indicating that significant changes in the cells have occurred.


Nov 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·J GilE R Weibel
Jul 1, 1977·Microvascular Research·O D WangensteenP Savaryn
Jun 1, 1971·The Journal of Cell Biology·Q Bone, E J Denton
Nov 1, 1967·Journal of Theoretical Biology·J A Johnson, T A Wilson
Dec 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·D WangensteenE R Weibel


Feb 5, 2010·American Journal of Respiratory and Critical Care Medicine·Connie C W HsiaATS/ERS Joint Task Force on Quantitative Assessment of Lung Structure
Jul 29, 2006·Journal of Microscopy·M Ochs
Jan 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jan Philipp Schneider, M Ochs
Sep 7, 2019·Acta Neuropathologica Communications·Whitney C McFaddenAndrew T McKenzie
Jan 1, 1984·The American Journal of Physiology·J Gil, J M McNiff

Related Concepts

Osmium Tetroxide
Water-Electrolyte Balance

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