Effects of GM-CSF deprivation on precursors of granulocytes and macrophages

Journal of Cellular Physiology
D Metcalf, S Merchav

Abstract

Culture of C57BL bone marrow cells in the absence of GM-CSF led to a loss of recoverable granulocyte-macrophage colony-forming cells of 2% per hour. The rate of loss of progenitor cells in cultures of CBA fetal liver cells was 5-6% per hour. Surviving colony-forming cells exhibited a normal responsiveness to GM-CSF but generated smaller colonies than normal when subsequently stimulated by GM-CSF. Transfer of washed individual day-3 granulocyte-macrophage colony cells to cultures lacking GM-CSF indicated that most cells were unable to survive or proliferate in the absence of GM-CSF. Death of transferred cells was rapid and invariable when the cells were from macrophage-forming colonies. However some cells from 40-70% of granulocyte-forming colonies were able to undergo one or two divisions in the absence of GM-CSF. This phenomenon was seen most often with cells from colonies where matching colony cells exhibited a higher-than-average proliferative capacity in parallel stimulated cultures. The results indicate the difficulty that will be encountered in obtaining valid metabolic data from unstimulated populations of granulocyte-macrophage precursor cells. The ability of some granulocyte precursor cells to exhibit limited prolifera...Continue Reading

References

Oct 1, 1977·Annals of Internal Medicine·D Metcalf
Jun 1, 1974·Journal of Cellular Physiology·H S Lin, C C Stewart
Nov 15, 1980·International Journal of Cancer. Journal International Du Cancer·A W Burgess, D Metcalf
Jun 1, 1982·Journal of Cellular Physiology·D Metcalf, A W Burgess
Sep 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M WattF L Battye

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Citations

Sep 1, 1992·Cancer Metastasis Reviews·N J McCarthyG T Williams
Jan 1, 1990·Biotherapy·W E Fibbe, J H Falkenburg
Jan 1, 1987·Immunologic Research·D E WilliamsH E Broxmeyer
Oct 25, 1991·Journal of Immunological Methods·A EischenF Oberling
Apr 6, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
Jan 29, 1999·Experimental Hematology·M OsadaT Nagasawa
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D H PluznikP D Noguchi
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D E WilliamsH E Broxmeyer
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Metcalf, N A Nicola
Jan 1, 1995·Mediators of Inflammation·C Haanen, I Vermes
Mar 1, 1987·Blut·E Platzer, J R Kalden
May 1, 1986·International Journal of Cell Cloning·J L Spivak
Apr 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M G CecchiniP H Cooper
Aug 15, 1982·International Journal of Cancer. Journal International Du Cancer·D Metcalf
Mar 25, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Palash BhattacharyaBellur S Prabhakar
Mar 1, 1984·Immunology Today·N A Nicola, M Vadas
Feb 1, 1987·Immunology and Cell Biology·D Metcalf
Mar 12, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Metcalf
May 22, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Metcalf
Jan 1, 1988·European Journal of Haematology. Supplementum·B T MortensenA N Jensen
May 16, 1988·The Medical Journal of Australia·D Metcalf
Dec 17, 1989·Biochimica Et Biophysica Acta·J H Pierce

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