Effects of gonadotropins and granulosa cell secretions on the maturation and fertilization of rat oocytes in vitro

Molecular Reproduction and Development
B C Vanderhyden, D T Armstrong

Abstract

Fully grown germinal vesicle-stage oocytes are induced to resume meiosis and acquire the capacity to undergo fertilization in response to a surge of gonadotropins. The present study examined possible direct and indirect roles of gonadotropins in the maturation and fertilization of rat oocytes by determining 1) the effect of exogenous administration of gonadotropins (priming) to immature rats prior to oocyte collection on the capacity of oocytes to undergo maturation and fertilization in vitro, 2) the effect of follicle-stimulating hormone (FSH) in the maturation media on the resumption of meiosis and subsequent capacity of oocytes to undergo fertilization, and 3) the capacity of oocytes to undergo maturation and fertilization following culture in preovulatory follicular fluid or in conditioned media obtained from gonadotropin-stimulated granulosa cell (GC) cultures. In the first experiment, oocytes from unprimed rats underwent spontaneous meiotic maturation in vitro and 17% underwent subsequent fertilization. Priming increased the proportion of oocytes undergoing fertilization. Maturation of oocytes in media supplemented with various concentrations of FSH or for various lengths of time (6-16 h) in medium with 500 ng FSH/ml indi...Continue Reading

References

Nov 1, 1977·The Journal of Endocrinology·A TsafririH R Lindner
Aug 15, 1976·Life Sciences·R B Gwatkin, O F Andersen
Oct 1, 1975·Journal of Reproduction and Fertility·T Iwamatsu, R Yanagimachi
Jun 1, 1976·Developmental Biology·R A Sorensen, P M Wassarman
May 1, 1989·Biology of Reproduction·B C VanderhydenD T Armstrong
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S M DownsJ J Eppig
Jan 1, 1985·Acta Physiologica Scandinavica·O M SejerstedF Kiil
Jul 1, 1986·Journal of Reproduction and Fertility·B C VanderhydenD T Armstrong
Oct 1, 1972·Journal of Reproduction and Fertility·A TsafririS A Lamprecht
Aug 1, 1972·The Journal of Endocrinology·C G ScanesJ M Dodd
Jul 1, 1972·Endocrinology·S Stoklowsowa, A V Nalbandov
Sep 1, 1972·The Journal of Endocrinology·K Szybek
Apr 1, 1984·Developmental Biology·A C Schroeder, J J Eppig
Jan 1, 1984·Differentiation; Research in Biological Diversity·E Sato, S S Koide
Oct 31, 1935·The Journal of Experimental Medicine·G Pincus, E V Enzmann

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Citations

Feb 1, 1996·Molecular Reproduction and Development·L MengD T Armstrong
Apr 1, 1996·Molecular Reproduction and Development·R S IsmailB C Vanderhyden
Feb 1, 1992·Molecular Reproduction and Development·A I Younis, B G Brackett
Mar 1, 1993·Molecular Reproduction and Development·K E Mate, J C Rodger
Jun 1, 1994·Molecular Reproduction and Development·S Coskun, Y C Lin
Apr 1, 1997·Journal of Assisted Reproduction and Genetics·K L DurinziS E Lanzendorf
Nov 1, 1996·Zygote : the Biology of Gametes and Early Embryos·S Cecconi, R Colonna
Jan 1, 1991·Annals of the New York Academy of Sciences·D T ArmstrongF Khamsi
Nov 15, 2006·Animal Reproduction Science·C E FarinS Kennedy-Stoskopf
Apr 1, 1994·Molecular Reproduction and Development·R D SchrammB D Bavister
Feb 4, 2006·Reproductive Biomedicine Online·Naiqing ChenSoon-Chye Ng

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