PMID: 28513459May 18, 2017Paper

Effects of Granulocyte Colony-Stimulating Factor on Opsonin Receptor Expression and Neutrophil Antibacterial Activity in a Mouse Model of Severe Acute Pancreatitis

Postȩpy higieny i medycyny doświadczalnej
Tuo Hong-FangZhang Wan-Xing

Abstract

The antimicrobial function of neutrophils, which is dependent on opsonin receptors, deteriorates in severe acute pancreatitis (SAP). Granulocyte colony-stimulating factor (G-CSF) putatively enhanced levels of the opsonin receptors CD11b and CD32/16 in healthy human subjects, and provided protection against infection in animal models of SAP. A statistically convincing study of the effect of G-CSF on CD32/16 expression in an SAP model is lacking. We used a mouse model of SAP to investigate the association between G-CSF administration and CD32/16 levels on neutrophils and bacterial translocation. G-CSF or saline was subcutaneously injected into SAP-induced mice. The pancreases were histologically examined, and leukocytes were stained to count neutrophils. The expression of CD11b and CD32/16 on neutrophils was measured by flow cytometry, and bacterial translocation was observed by bacterial culture. The numbers of CD11b and CD32/16-positive neutrophils were significantly elevated in the SAP mice treated with G-CSF, and the mean fluorescence intensities of these receptors on neutrophils were significantly elevated. Bacterial translocations to cavity organs were suppressed from 17% to 6% by G-CSF treatment. Our results indicated that...Continue Reading

References

Mar 1, 1997·European Journal of Haematology·M HöglundP Venge
Jul 17, 1998·Current Opinion in Hematology·T Hartung
Feb 27, 2003·The Journal of Pharmacology and Experimental Therapeutics·Vinay S BansalPatricia A Detmers
Aug 15, 2003·The Cochrane Database of Systematic Reviews·M J Burton, C J Dorée
Oct 30, 2003·The Cochrane Database of Systematic Reviews·A C ChengB J Currie

❮ Previous
Next ❯

Citations

Jul 11, 2019·Oncology Letters·Katarzyna JakubowskaWaldemar Famulski

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.