Effects of Granulocyte-Macrophage Colony-Stimulating Factor on Neuronal Senescence in Ultraviolet Irradiated Skin

The Journal of Craniofacial Surgery
Kyung-Chul MoonSeung-Kyu Han

Abstract

Ultraviolet (UV) irradiation affects neuronal structures of the skin and accelerates skin aging. Cytokine cascades in keratinocytes after UV irradiation may result in a paracrine inhibitory effect on nerve cells. The purpose of the present study was to determine the direct effect of cytokines induced by UV radiation on nerve cells in terms of neuronal senescence. Our group performed a preliminary study to determine cytokines induced in UV-irradiated keratinocytes. Among 40 cytokines studied, granulocyte-macrophage colony-stimulating factor (GM-CSF) was increased 4-fold in inflammation antibody array. The GM-CSF was added to cultured human neuroblastoma cells. To evaluate the effect of cellular senescence, the authors performed real-time polymerase chain reaction (RT-PCR), western blot, immunocytochemical, and phase-contrast microscopic evaluations. Expression levels of matrix metallopeptidase-9 (MMP-9), nuclear factor kappa-light-chain-enhancer of activated B cells 1 (NF-κB1), inducible nitric oxide synthase (iNOS), and interleukin β1 (IL-β1) were assessed by RT-PCR. Expression levels of AAP and beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) related to formation of beta-amyloid were evaluated by western blot anal...Continue Reading

References

Nov 1, 1991·The Journal of Cell Biology·M YaarB A Gilchrest
Dec 31, 1997·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C BayerlE G Jung
Dec 29, 1998·Archives of Neurology·J C McArthurJ W Griffin
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R G NickelS K Huang
May 24, 2000·Photodermatology, Photoimmunology & Photomedicine·L Misery
Mar 7, 2001·Antioxidants & Redox Signaling·B KaltschmidtC Kaltschmidt
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·A MiceraF Levi-Schaffer
Jul 4, 2001·Nature Reviews. Neuroscience·V W YongD R Edwards
Nov 7, 2001·Clinical and Experimental Dermatology·M Yaar, B A Gilchrest
Nov 16, 2002·The Journal of Laboratory and Clinical Medicine·Tadashi KohyamaStephen I Rennard
Jan 27, 2004·The American Journal of Pathology·Hiroaki FukumotoMichael C Irizarry
Nov 11, 2005·The British Journal of Dermatology·M ToyodaM Morohashi
Apr 4, 2006·Current Opinion in Rheumatology·Norihiro Nishimoto
Jun 29, 2006·Annals of the New York Academy of Sciences·Jerry L McCullough, Kristen M Kelly
Oct 4, 2006·Physiological Reviews·Dirk RoostermanMartin Steinhoff
Aug 10, 2010·Biological Psychiatry·Walter SwardfagerNathan Herrmann
Aug 27, 2013·Methods in Molecular Biology·Jane Kovalevich, Dianne Langford
Oct 6, 2014·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Marcelo Augusto Marretto EsquisattoLaurecir Gomes
Dec 4, 2015·Nature Reviews. Rheumatology·Ian P Wicks, Andrew W Roberts
Mar 30, 2016·Journal of Tissue Viability·Desmond J Tobin
Nov 11, 2017·Advances in Experimental Medicine and Biology·Luisa ChristensenElma Baron

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-Arrhythmic Drug Therapies

Anti-arrhythmic drugs are used to prevent abnormal heart rhythms. These medications are used in conditions including, ventricular tachycardia, ventricular fibrillation and atrial fibrillation. Discover the latest research on anti-arrhythmic drug therapies here.