Effects of Group 3 LEA protein model peptides on desiccation-induced protein aggregation.

Biochimica Et Biophysica Acta
Takao FurukiMinoru Sakurai

Abstract

Group 3 late embryogenesis abundant (G3LEA) proteins have amino acid sequences with characteristic 11-mer motifs and are known to reduce aggregation of proteins during dehydration. Previously, we clarified the structural and thermodynamic properties of the 11-mer repeating units in G3LEA proteins using synthetic peptides composed of two or four tandem repeats originating from an insect (Polypedilum vanderplanki), nematodes and plants. The purpose of the present study is to test the utility of such 22-mer peptides as protective reagents for aggregation-prone proteins. For lysozyme, desiccation-induced aggregation was abrogated by low molar ratios of a 22-mer peptide, PvLEA-22, derived from a P. vanderplanki G3LEA protein sequence. However, an unexpected behavior was noted for the milk protein, α-casein. On drying, the resultant aggregation was significantly suppressed in the presence of PvLEA-22 with its molar ratios>25 relative to α-casein. However, when the molar ratio was <10, aggregation occurred on addition of PvLEA-22 to aqueous solutions of α-casein. Other peptides derived from nematode, plant and randomized G3LEA protein sequences gave similar results. Such an anomalous solubility change in α-casein was shown to be due t...Continue Reading

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Citations

May 2, 2016·Biochimica Et Biophysica Acta·Takao Furuki, Minoru Sakurai
Jun 25, 2016·International Journal of Biological Macromolecules·Tingzhang HuXiaoyun Huang
May 19, 2019·Protein Science : a Publication of the Protein Society·Sharall R PalmerSteffen P Graether
Jul 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Robin Rajan, Kazuaki Matsumura
Nov 6, 2020·Cell Communication and Signaling : CCS·Cherie Hesgrove, Thomas C Boothby
Dec 7, 2018·Biochemistry and Biophysics Reports·Takao FurukiMinoru Sakurai
Sep 21, 2013·Plant Physiology·Stephanie L HughesSteffen P Graether
Sep 13, 2014·The Journal of Physical Chemistry. B·Robert M CulikFeng Gai

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