Effects of growth factors and hormones on growth and morphological differentiation of human breast epithelial cells within collagen gel in serum-free medium

Japanese Journal of Cancer Research : Gann
K TakahashiT Ono

Abstract

When human epithelial cells that had grown out from either carcinoma or histologically non-malignant breast tissues were seeded within type I collagen gels in serum-free medium, they successively grew and protruded many radial duct-like extensions with lumina. Separate deletion of each of the supplements from the medium showed that growth as well as morphological differentiation of carcinoma-derived cells were prevented in the absence of epidermal growth factor (EGF) or hydrocortisone. Removal of insulin or ethanolamine plus phosphoethanolamine caused a significant inhibition of cell growth without interfering with the morphological differentiation. Contrary to the case with carcinoma-derived cells, both growth and morphological differentiation of epithelial cells derived from non-malignant breast tissues were prevented when EGF, hydrocortisone or insulin was absent. Removal of each of the other supplements (except for transferrin), including ethanolamine plus phosphoethanolamine, prolactin, or prostaglandin, caused a significant inhibition of cell growth with no apparent inhibition of morphological differentiation. The present results suggest that human epithelial cells derived from either carcinoma or histologically non-malig...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J YangS Nandi
May 15, 1989·International Journal of Cancer. Journal International Du Cancer·K TakahashiT Ono
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L HammondM R Stampfer
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J YangS Nandi
May 1, 1980·In Vitro·M StampferA J Hackett

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Citations

Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Thorarinn GudjonssonOle William Petersen
Apr 15, 1999·Journal of the National Cancer Institute·S E HankinsonF E Speizer
Feb 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N P PerusingheB A Gusterson
May 25, 2012·Clinical Breast Cancer·Yupeng LiuYashuang Zhao
Mar 12, 1992·International Journal of Cancer. Journal International Du Cancer·C J OrmandyR L Sutherland
Dec 31, 1990·Biochemical and Biophysical Research Communications·K TakahashiT Ono

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