Effects of growth factors on phosphatidylinositol-3 kinase in astroglial cells

Journal of Neuroscience Research
M PomeranceM Pierre

Abstract

Growth factors differently regulate astroglial cell differentiation and proliferation. In an effort to understand the early intracellular events promoted by growth factors in astroglial cells, we have determined the effects of insulin-like growth factor I (IGF1), insulin, platelet-derived growth factor (PDGF), epidermal growth factor (EGF) and fibroblast growth factors (FGFs) on phosphatidylinositol-3 kinase (PI(3)-kinase). In astroglial cells cultured in serum-free medium, IGF1, PDGF, and EGF, which stimulate cell proliferation, increased PI(3)-kinase activity immunoprecipitated with anti-phosphotyrosine antibodies as shown by thin layer chromatography and high performance liquid chromatography. FGFa and FGFb, which strongly stimulate proliferation, glutamine synthetase, and deiodinase activities and modify cell morphology, have no effect on PI(3)-kinase activity. Addition of 1 nM PDGF, 10 nM IGF1, or 100 nM EGF to the culture medium rapidly stimulated PI(3)-kinase activity which declined slowly after 2 min. The stimulation of PI(3)-kinase increased with growth factor concentration. The maximum increase in PI(3)-kinase activity occurred with 50 nM IGF1, 1 nM PDGF, or 100 nM EGF. Since insulin was active only at high concentrat...Continue Reading

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Citations

Sep 5, 2002·Journal of Cellular Biochemistry·Claudia GentiliAna Russo De Boland
Jan 13, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R AvolaB Marchetti
Aug 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Erin E Rodgers, Anne B Theibert
May 28, 2009·Journal of Neurotrauma·Jeong-Sun ChoiMun-Yong Lee
Nov 25, 2003·Journal of Neuroscience Research·Esther López-BayghenArturo Ortega
Feb 24, 2007·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·S FernandezI Torres-Aleman
Jul 7, 2005·Journal of Neurochemistry·Céline VermeirenEmmanuel Hermans
Feb 13, 2020·Journal of Translational Medicine·Inma Castilla-CortázarLuis Espinosa

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