Effects of growth hormone, insulin-like growth factor I, triiodothyronine, thyroxine, and cortisol on gene expression of carbohydrate metabolic enzymes in sea bream hepatocytes

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
L Y Leung, Norman Y S Woo

Abstract

The present study investigated the regulatory effects of growth hormone (GH), human insulin-like growth factor I (hIGF-I), thyroxine (T(4)), triiodothyronine (T(3)) and cortisol, on mRNA expression of key enzymes involved in carbohydrate metabolism, including glucokinase (GK), glucose-6-phosphatase (G6Pase), glycogen synthase (GS), glycogen phosphorylase (GP) and glucose-6-phosphate dehydrogenase (G6PDH) in hepatocytes isolated from silver sea bream. Genes encoding GK, G6Pase, GS and GP were partially cloned and characterized from silver sea bream liver and real-time PCR assays were developed for the quantification of the mRNA expression profiles of these genes in order to evaluate the potential of these carbohydrate metabolic pathways. GK mRNA level was elevated by GH and hIGF-I, implying that GH-induced stimulation of GK expression may be mediated via IGF-I. GH was found to elevate GS and G6Pase expression, but reduce G6PDH mRNA expression. However, hIGF-I did not affect mRNA levels of GS, G6Pase and G6PDH, suggesting that GH-induced modulation of GS, G6Pase and G6PDH expression levels is direct, and occurs independently of the action of IGF-I. T(3) and T(4) directly upregulated transcript abundance of GK, G6Pase, GS and GP. ...Continue Reading

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Citations

Jun 19, 2012·Physiological and Biochemical Zoology : PBZ·Kathleen M GilmourThomas W Moon
Aug 21, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mei-Qin ZhuoQi-Liang Chen
Mar 10, 2015·General and Comparative Endocrinology·Yu WangShicui Zhang
Jul 14, 2019·Fish Physiology and Biochemistry·Michael J LawrenceSteven J Cooke
Nov 27, 2020·Frontiers in Endocrinology·Cole K Deal, Helene Volkoff

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