Effects of growth medium on matrix-assisted laser desorption-ionization time of flight mass spectra: a case study of acetic acid bacteria

Applied and Environmental Microbiology
Anneleen D WiemePeter Vandamme

Abstract

The effect of the growth medium used on the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra generated and its consequences for species and strain level differentiation of acetic acid bacteria (AAB) were determined by using a set of 25 strains. The strains were grown on five different culture media that yielded a total of more than 600 mass spectra, including technical and biological replicates. The results demonstrate that the culture medium can have a profound effect on the mass spectra of AAB as observed in the presence and varying signal intensities of peak classes, in particular when culture media do not sustain optimal growth. The observed growth medium effects do not disturb species level differentiation but strongly affect the potential for strain level differentiation. The data prove that a well-constructed and robust MALDI-TOF mass spectrometry identification database should comprise mass spectra of multiple reference strains per species grown on different culture media to facilitate species and strain level differentiation.

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Citations

Jun 16, 2014·International Journal of Food Microbiology·Anneleen D WiemePeter Vandamme
Dec 18, 2015·Future Microbiology·Edoardo CarrettoDaniela Barbarini
May 17, 2017·Mass Spectrometry Reviews·Todd R Sandrin, Plamen A Demirev
Mar 18, 2015·Applied Microbiology and Biotechnology·Dominik ZieglerXavier Perret
Sep 6, 2019·Analytical and Bioanalytical Chemistry·Christoph MörtelmaierImke Mulder
Jan 8, 2020·International Journal of Systematic and Evolutionary Microbiology·Guillaume TahonAnne Willems
Jun 13, 2020·Archives of Microbiology·Livia M R VidalCristiane C Thompson
Aug 8, 2020·Frontiers in Microbiology·Eliza DepoorterPeter Vandamme
Oct 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michał ZłochBogusław Buszewski
May 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Kieran M LynchElke K Arendt
Mar 22, 2020·International Journal of Food Microbiology·Charalampos GantziasPeter Vandamme
May 5, 2021·Systematic and Applied Microbiology·Angélique RatAnne Willems
May 31, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sachio TsuchidaFumio Nomura
Sep 5, 2021·Antonie van Leeuwenhoek·Peter VandammeDaniel Goldenberger

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