Effects of Gui Zhi Ma Huang Ge Ban Tang on the TLR7 Pathway in Influenza Virus Infected Mouse Lungs in a Cold Environment

Evidence-based Complementary and Alternative Medicine : ECAM
Hong-Qiong QinZhen-You Jiang

Abstract

We wished to investigate the effects of the traditional Chinese medicine Gui Zhi Ma Huang Ge Ban Tang on controlling influenza A virus (IAV) infection and improving inflammation in mouse lungs. Mice were maintained in normal and cold environments and infected with IAV by intranasal application, respectively. Real-time quantitative polymerase chain reaction was used to measure mRNA expression of TLR7, myeloid differentiation primary response 88 (MyD88), and nuclear factor-kappa B (NF-κB)p65 in the TLR7 signaling pathway and virus replication in lungs. Western blotting was used to measure expression levels of TLR7, MyD88, and NF-κB p65 proteins. Flow cytometry was used to detect the proportion of T-helper (Th)1/Th2 and Th17/T-regulatory (Treg) cells. Application of Gui Zhi Ma Huang Ge Ban Tang in influenza-infected mice in a cold environment showed (i) downregulation of TLR7, MyD88, and NF-κBp65; (ii) inhibition of transcriptional activities of promoters coding for TLR7, MyD88, and NF-κBp65; (iii) reduction in the proportion of Th1/Th2 and Th17/Treg cells. Gui Zhi Ma Huang Ge Ban Tang had a good therapeutic effect on mice infected with IAV, especially in the cold environment. It could reduce lung inflammation in mice significantl...Continue Reading

References

Dec 28, 2006·Journal of Biochemistry·Taro Kawai, Shizuo Akira
May 7, 2010·The New England Journal of Medicine·Edgar BautistaKarl G Nicholson
Dec 16, 2011·Virology Journal·Wen HeBin Gao
Jun 26, 2012·Respiratory Research·Lennart GreiffHenrik Widegren
Sep 21, 2013·Critical Reviews in Oncology/hematology·Sara HarsiniNima Rezaei
Mar 1, 2014·The Pediatric Infectious Disease Journal·Sandra S ChavesUNKNOWN Influenza Hospitalization Surveillance Network
Mar 21, 2015·PloS One·Alexis BoneparthAnne Davidson
Aug 1, 2015·Phytochemistry·Ruchi Badoni SemwalAlvaro M Viljoen
Aug 13, 2015·Molecular Immunology·Mario GalganiGiuseppe Matarese
Jun 2, 2016·Health Technology Assessment : HTA·Carl J HeneghanTom Jefferson
Mar 1, 2017·Archives of Virology·Shang-Hui LiaoPei-Ping Xu
Mar 30, 2017·Methods in Molecular Biology·Robert A BensonMegan K L MacLeod
May 4, 2017·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Changchong LiWeixi Zhang
Aug 12, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Hui-Hua ChenRong Lu

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Citations

Jul 18, 2020·Medicinal Research Reviews·Zhi-Jun ZhangRong-Tao Li
Nov 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao ZhengZhenyou Jiang

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Methods Mentioned

BETA
PCRs
protein assay
electrophoresis
flow cytometry

Software Mentioned

ImageJ
SPSS

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