Effects of guluronic acid (G2013) on SHIP1, SOCS1 induction and related molecules in TLR4 signaling pathway

International Immunopharmacology
Seyed Shahabeddin Mortazavi-JahromiAbbas Mirshafiey

Abstract

This research aimed to study the anti-inflammatory and immunomodulatory effects of guluronic acid (G2013) on gene expression of TLR4, MyD88, SHIP1, SOCS1, NF-κB, and assessment of the level of IL-1β as a pro-inflammatory cytokine in HEK-Blue hTLR4 cell line. The cytotoxicity of G2013 was assessed by the MTT assay. The mRNA expression levels of the mentioned genes were measured by qRT-PCR. IL-1β concentration in culture media was determined using ELISA method. MTT assay demonstrated that G2013 (before the concentration of 125μg/ml) had no cytotoxic effect on HEK-Blue hTLR4 cells. Our results indicated that the low and high doses of this drug could significantly reduce the gene expression of TLR4 and MyD88, as compared to the control group (p<0.05). Moreover, it was found that the low dose of this drug could significantly increase the gene expression of SHIP1 and SOCS1, as compared to the control group (p<0.05). Furthermore, the study findings revealed that the level of NF-κB gene expression significantly reduced, in both doses of G2013 compared to the control group (p<0.05, p<0.01, respectively). Our data showed that the level of IL-1β in culture media decreased by both doses of this drug in comparison to control group (p<0.05)....Continue Reading

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Citations

Jan 10, 2019·Immunopharmacology and Immunotoxicology·Shahin Khadem AzarianAbbas Mirshafiey
Oct 9, 2019·Immunopharmacology and Immunotoxicology·Seyedeh Masoomeh NoorbakhshAbbas Mirshafiey
Oct 18, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Seyed S Mortazavi-JahromiAbbas Mirshafiey
Mar 4, 2020·Marine Drugs·Maochen XingShuliang Song
Oct 27, 2021·Clinical and Experimental Pharmacology & Physiology·Zahra BagherianMohammad M Farajollahi

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