Effects of H2O2 on membrane potential and [Ca2+]i of cultured rat arterial smooth muscle cells

Biochemical and Biophysical Research Communications
P Krippeit-DrewsF Lang

Abstract

The effect of 1 mmol/l H2O2 was studied on the membrane potential and [Ca2+]i with microelectrodes and the fura-2 technique, respectively. H2O2 induced a biphasic increase in [Ca2+]i with a fast transient peak and a subsequent plateau. H2O2 also led to a biphasic hyperpolarization of the cells with a similar time course. This was followed by a slight depolarization after wash-out of H2O2. External Ca2+ free solutions and treatment with the Ca2+ ionophore A23187 (1 mumol/l) abolished the effect of H2O2 on [Ca2+]i and almost entirely reduced the effect on the membrane potential. Phenylephrine (10 mumol/l) or A23187 also induced very similar biphasic hyperpolarizations of the membrane as H2O2 which were fully reversible after wash-out. It is concluded that H2O2 hyperpolarizes the membrane by opening of Ca2+ dependent K+ channels.

Citations

Sep 7, 2006·Molecular and Cellular Biochemistry·I BejaranoJ A Pariente
Sep 24, 1998·The American Journal of Physiology·R S Barlow, R E White
Feb 11, 1998·Physiological Reviews·F M Faraci, D D Heistad
Apr 15, 2005·Redox Report : Communications in Free Radical Research·Yi Zheng, Xun Shen
Mar 21, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mo-Jun LinJames S K Sham
Nov 5, 2010·Diabetes, Obesity & Metabolism·J Pi, S Collins
Mar 4, 2006·Experimental Biology and Medicine·Noelia Ardanaz, Patrick J Pagano
Dec 16, 1998·The Journal of Physiology·P Krippeit-DrewsG Drews
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·W HeberleinH Mairbäurl
Jun 19, 2013·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Shinkyu ChoiSuk Hyo Suh
Jul 1, 1997·British Journal of Pharmacology·V BauerY Ito
Feb 8, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·María P GranadosJosé A Pariente
Nov 17, 2007·Molecular and Cellular Biochemistry·Miriam S Giambelluca, Oscar A Gende
Nov 14, 1998·The American Journal of Physiology·C G SobeyF M Faraci
May 26, 1998·European Journal of Pharmacology·Z W YangB M Altura
Jan 22, 1998·Free Radical Biology & Medicine·I A GamaleyI V Klyubin
Feb 13, 2001·European Journal of Pharmacology·S FujimotoT Itoh
Jul 13, 2000·Free Radical Biology & Medicine·H Wang, J A Joseph
Jul 20, 1999·Kidney International·J GloyH Pavenstädt

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