Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles

Journal of Proteomics
Rita MotaPaula Tamagnini

Abstract

The effects of several heavy metals on the growth/survival, EPS production, ultrastructure and protein profiles of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110 were evaluated. Our results clearly show that each heavy metal affects the cells in a particular manner, triggering distinctive responses. Concerning chronic exposure, cells were more affected by Cu(2+) followed by Pb(2+), Cd(2+), and Li(+). The presence of metal leads to remarkable ultrastructural changes, mainly at the thylakoid level. The comparison of the proteomes (iTRAQ) allowed to follow the stress responses and to distinguish specific effects related to the time of exposure and/or the concentration of an essential (Cu(2+)) and a non-essential (Cd(2+)) metal. The majority of the proteins identified and with fold changes were associated with photosynthesis, CO2 fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolism. Moreover, our results indicate that during chronic exposure to sub-lethal concentrations of Cu(2+), the cells tune down their metabolic rate to invest energy in the activation of detoxification mechanisms, which eventually result in a remarkable recovery....Continue Reading

References

Jan 1, 1971·Archiv für Mikrobiologie·J C Meeks, R W Castenholz
Feb 1, 1995·Journal of Industrial Microbiology·J S Turner, N J Robinson
Jul 28, 1999·Applied Microbiology and Biotechnology·D H Nies
Sep 4, 2001·Biotechnology & Genetic Engineering Reviews·P LiZ Liu
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·H XuW Vermaas
Feb 22, 2003·Acta Crystallographica. Section D, Biological Crystallography·David R HallWilliam N Hunter
Jun 28, 2003·FEMS Microbiology Reviews·Jennifer S CavetNigel J Robinson
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Jan 30, 2004·Environment International·Anushree Malik
Feb 6, 2004·British Medical Bulletin·Lars Järup
Apr 27, 2004·Bulletin of Environmental Contamination and Toxicology·L Y HengM Idris
May 17, 2005·Current Medicinal Chemistry·M ValkoM T D Cronin
Oct 20, 2005·Accounts of Chemical Research·Stephen TotteyNigel J Robinson
Nov 1, 2005·Bioresource Technology·Amit Parikh, Datta Madamwar
Jul 11, 2006·International Journal of Environmental Research and Public Health·Ali B IshaquePaul B Tchounwou
Aug 9, 2006·Critical Reviews in Microbiology·Mafalda S Baptista, M Teresa Vasconcelos
Nov 29, 2007·BMC Genomics·Balakrishnan PriyaGopalakrishnan Subramanian
Jan 15, 2008·Briefings in Functional Genomics & Proteomics·Berta De la CerdaMiguel A De la Rosa
Mar 11, 2008·Applied and Environmental Microbiology·Ernesto MichelettiRoberto De Philippis
Oct 7, 2008·FEMS Microbiology Reviews·Amel LatifiCheng-Cai Zhang
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Oded LewinsonDouglas C Rees
Apr 9, 2009·FEBS Letters·Ornella CastielliMiguel A De la Rosa
Mar 9, 2010·Annual Review of Biochemistry·Nigel J Robinson, Dennis R Winge
Dec 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Steve TotteyNigel J Robinson
May 18, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·L HudekM Leigh Ackland
Oct 18, 2012·Plant Signaling & Behavior·Luis López-MauryFrancisco J Florencio

❮ Previous
Next ❯

Citations

Oct 17, 2015·International Journal of Molecular Sciences·Krystian MiazekDorothee Goffin
Jun 17, 2015·Environmental Science and Pollution Research International·De-ju CaoXiao-dong Shi
Jul 5, 2016·Frontiers in Microbiology·Joaquín Giner-LamiaPaulo Oliveira
Feb 12, 2019·Critical Reviews in Food Science and Nutrition·Mayara C S BarcelosGustavo Molina
May 6, 2019·Metabolites·Nattaphorn BuayamChayakorn Pumas
May 14, 2018·Environmental Science and Pollution Research International·Li ShenWeimin Zeng
Nov 5, 2019·Environmental Science and Pollution Research International·Emilie LoustauJean-Luc Rols
Mar 13, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ayushee Rath, Anath Bandhu Das

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.