Effects of hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by twelve methods

Clinica Chimica Acta; International Journal of Clinical Chemistry
Curt RohlfingRandie R Little

Abstract

Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results. We assessed whether they affect results obtained with 12 current assay methods. Hemoglobin AA (HbAA), HbAC, HbAD Punjab, HbAE and HbAS samples were analyzed on one enzymatic, nine ion-exchange HPLC and two Capillary Electrophoresis methods. Trinity ultra(2) boronate affinity HPLC was the comparative method. An overall test of coincidence of least-squared linear regression lines was performed to determine if HbA1c results were statistically significantly different from those of HbAA samples. Clinically significant interference was defined as >7% difference from HbAA at 6 or 9% HbA1c compared to ultra(2) using Deming regression. All methods showed statistically significant effects for one or more variants. Clinically significant effects were observed for the Tosoh G8 variant mode and GX (all variants), GX V1.22 (all but HbAE) and G11 variant mode (HbAC). All other methods (Abbott Architect c Enzymatic, Bio-Rad D-100, Variant II NU and Variant II Turbo 2.0, Menarini HA-8180T thalassemia mode and HA-8180V variant mode, Sebia Capillarys 2 and Capillarys 3) showed no clinically significant differences. Several methods showed clinically significant...Continue Reading

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Citations

Apr 27, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kuanysh KabytaevRandie R Little
May 20, 2017·Scandinavian Journal of Clinical and Laboratory Investigation·Yan WangPeng Jiang
Apr 26, 2017·Endocrinología, Diabetes Y Nutrición·Pedro José Pinés CorralesÁngela Vicente Albiñana
Apr 19, 2018·Journal of Clinical Laboratory Analysis·Sophie Van AelstDavy Kieffer
Jul 12, 2018·Scandinavian Journal of Clinical and Laboratory Investigation·Haneen KhashoggiSergio Bernardini
Sep 18, 2018·Journal of Clinical Laboratory Analysis·Anping XuLing Ji
Jun 9, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Elisa DaneseGiuseppe Lippi
Apr 23, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Erna Lenters-Westra, Emma English
Jan 1, 2020·PloS One·Fernando Chimela ChumeJoíza Lins Camargo
Jul 18, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Anping XuLing Ji
Jul 1, 2020·The Journal of Applied Laboratory Medicine·Ghaith AltawallbehAnthony A Killeen
Dec 15, 2020·Scandinavian Journal of Clinical and Laboratory Investigation·Jianfeng XuYukui Deng
Aug 27, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anping XuLing Ji
Aug 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Curt RohlfingRandie R Little

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