Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm

Theriogenology
K H Lu, G E Seidel

Abstract

The objective was to determine the optimal concentration of heparin for sperm capacitation, as well as the optimal sperm concentration for in vitro fertilization using flow cytometrically-sorted sperm from individual bulls. A total of 5327 bovine oocytes and sperm from four bulls were examined. Oocytes from slaughterhouse ovaries were matured in TCM199 for 22-24 h. Flow cytometrically-sorted sperm as well as unsorted control sperm from the same bulls were cryopreserved. For sperm from each of the four bulls, oocytes were inseminated in a three-by-three factorial design plus one control group (three heparin concentrations: 0, 2, and 10 microg/ml and three sperm concentrations: 0.5 x 10(6), 1.5 x 10(6), and 4.5 x 10(6) ml(-1); 10 microg/ml of heparin and 1.5 x 10(6) ml(-1) of sperm were used for the unsorted control). Presumptive zygotes were cultured in chemically defined media, CDM-1 and CDM-2 for 52-54 h and 96 h, respectively. Samples of about 10 oocytes from each of the 10 treatment groups per replicate were fixed at 18-20 h after insemination to determine sperm pronuclei formation and polyspermy. Increased polyspermy resulted as heparin and sperm concentrations increased (P < 0.05). A higher rate of polyspermy was found in ...Continue Reading

References

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Citations

Oct 5, 2010·Veterinary Medicine International·Giorgio A PresicceFuliang Du
Apr 24, 2012·The Journal of Reproduction and Development·Yoo-Jin ParkMyung-Geol Pang
Jun 27, 2008·Zygote : the Biology of Gametes and Early Embryos·M MachatkovaK Hanzalova
Oct 25, 2008·Theriogenology·J XuF Du
Nov 14, 2008·Theriogenology·P BlondinW A King
Mar 8, 2007·Molecular Reproduction and Development·M Barceló-Fimbres, G E Seidel
Oct 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·M Barceló-FimbresG E Seidel
Jun 5, 2014·Reproduction in Domestic Animals = Zuchthygiene·X-M ZhaoH-B Zhu
Nov 3, 2005·Theriogenology·Matthew B WheelerDavid J Beebe
Feb 1, 2013·Asian-Australasian Journal of Animal Sciences·Eun Young KimSe Pill Park
Oct 21, 2016·The Journal of Reproduction and Development·Yasushi InabaTamás Somfai
Feb 7, 2017·The Journal of Reproduction and Development·Li-You AnFuliang DU
Nov 26, 2019·Animal : an International Journal of Animal Bioscience·L B FerréP J Ross
Jan 1, 2019·Reproduction, Fertility, and Development·M MikkolaJ Taponen
Mar 15, 2021·Reproduction in Domestic Animals = Zuchthygiene·Ivona TravnickovaMarie Machatkova

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