PMID: 7516428Jan 15, 1994Paper

Effects of heparin on excitation-contraction coupling in skeletal muscle toad and rat

The Journal of Physiology
G D LambD G Stephenson

Abstract

1. Intracellularly applied heparin was found to cause a novel, use-dependent block of excitation-contraction (E-C) coupling in skinned skeletal muscle fibres of the toad. After one to four depolarizations in the presence of 100 micrograms ml-1 heparin, no further depolarization-induced responses could be elicited, even though addition of caffeine or lowering [Mg2+] could still induce massive Ca2+ release. This effect could not be reversed by extensive wash-out of the heparin (> 15 min). 2. Heparin (100 micrograms ml-1) did not abolish subsequent depolarization-induced responses if applied while the voltage sensors were in either their resting or inactivated states, that is (a) while a fibre remained fully polarized, (b) when a fibre was already chronically depolarized or (c) after a fibre had been depolarized in the presence of D600 (gallopamil) and then repolarized. 3. When a toad fibre was depolarized in heparin, with the associated Ca2+ release blocked by the presence of 10 mM intracellular Mg2+, subsequent E-C coupling was abolished. Heparin did not interrupt E-C coupling when Ca2+ release was triggered in the absence of any depolarization, by either caffeine or low [Mg2+]. Thus, the opening of the Ca2+ release channels was...Continue Reading

Citations

Mar 3, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Isabelle MartellyBruno Constantin
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bradley S LaunikonisD George Stephenson
Jul 2, 2005·Journal of Cellular Physiology·Guido J JenniskensToin H van Kuppevelt
Feb 8, 2006·Canadian Journal of Physiology and Pharmacology·Marco S Lofrano-AlvesRosalvo T H Fogaça
Mar 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guido J JenniskensToin H van Kuppevelt
Nov 17, 2010·Fundamental & Clinical Pharmacology·Felipe Crescêncio LimaJosé Henrique Leal-Cardoso
Sep 26, 2000·American Journal of Physiology. Cell Physiology·G D LambD G Stephenson
Oct 1, 1996·Journal of Muscle Research and Cell Motility·M MartínezJ A Sánchez

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