PMID: 15248906Jul 14, 2004Paper

Effects of hepatitis B virus X gene on p21(WAF1) expression through p53-dependent and p53-independent pathways

Ai zheng = Aizheng = Chinese journal of cancer
Jing LinCan-Rong Ni

Abstract

It was reported that the hepatitis B virus X could inhibit the function of p53 and have contrary effects on p21(WAF1), a downstream gene of p53, but the mechanism is not clear up to now. So this study was designed to investigate HBx's effect on p21(WAF1) gene. After co-transfection of sense or antisense wild type p53 gene (wtp53) with HBx into SMMC-7721 and HBx alone transfection into other hepatoma cell lines with different endogenous status of p53, we evaluated the luciferase expression level under the p21(WAF1) promoter by detecting the luciferase activity and alteration of cell cycle in these transfected cell lines by flow cytometry. Expressions of p53 and p21 (WAF1) in hepatoma cells after transfected with HBx were also estimated by Western blot analysis. The luciferase activity (1.007+/-0.098) in SMMC-7721 cells cotransfected with HBx and sense-wtp53 was higher than that in cells with wtp53 gene alone (0.490+/-0.012, P< 0.05), and also was depressed in the other experimental groups transfected with HBx (P< 0.05). Western blot analysis showed that after transfected with HBx, p53 expression was elevated in all hepatoma cell lines with different endogenous status of p53, and the expression of p21(WAF1) and luciferase activit...Continue Reading

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