Effects of hepatitis E virus infection on interferon production via ISG15

World Journal of Gastroenterology : WJG
Min WangDeying Tian

Abstract

To assess the effects of hepatitis E virus (HEV) on the production of type I interferons (IFNs) and determine the underlying mechanisms. We measured the production of interferon (IFN)-alpha and -beta (-α/β) in genotype 3 HEV-infected C3A cells at different time points (0, 8, 12, 24, 48, 72 and 120 h) by enzyme-linked immunosorbent assay (ELISA). The expression levels of IFN-stimulated gene (ISG)15 in HEV-infected C3A cells at different time points were tested by western blotting. The plasmid-expressing open reading frame 3 (ORF3) or control plasmids (green fluorescent protein-expressing) were transfected into C3A cells, and the levels of IFN-α/β and ISG15 were evaluated, respectively. Furthermore, the plasmid-expressing ISG15 or small interfering RNA-inhibiting ISG15 was transfected into infected C3A cells. Then, the production of IFN-α/β was also measured by ELISA. We showed that genotype 3 HEV could enhance the production of IFN-α/β and induce elevation of ISG15 in C3A cells. HEV ORF3 protein could enhance the production of IFN-α/β and the expression of ISG15. Additionally, ISG15 silencing enhanced the production of IFN-α/β. Overexpression of ISG15 resulted in the reduction of IFN-α/β. HEV may promote production of IFN-α/β an...Continue Reading

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Citations

Aug 1, 2020·Vaccines·Sébastien LhommeJacques Izopet
Jun 8, 2021·World Journal of Gastroenterology : WJG·Yong-Lin Yang, Yu-Chen Nan

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Datasets Mentioned

BETA
55762

Methods Mentioned

BETA
transfection
protein assay
electrophoresis
PCR
ELISA
enzyme-linked immunosorbent assay
reverse transcription-PCR

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