Effects of hepatocyte growth factor on urokinase-type plasminogen activator (uPA) and uPA receptor in DU145 prostate cancer cells

International Journal of Andrology
Kenji NishimuraA Okuyama

Abstract

Urokinase-type plasminogen activator (uPA) and the uPA receptor (uPAR) are involved in a proteolytic cascade resulting of extracellular matrix degradation. Upstream, uPA and uPAR are regulated by various factors including hepatocyte growth factor (HGF), which stimulates the uPA/uPAR proteolytic system and increases invasion of cancers. We recently demonstrated that HGF induces invasion of DU145 prostate cancer cells into collagen gel matrix. We therefore examined effects of HGF on uPA and uPAR expression in DU145 cells. Effects of HGF on uPA expression in culture medium were determined by Western blotting and fibrin zymography, effects on uPAR expression in cell-associated protein were examined by Western blotting. HGF increased uPA and uPAR production in a dose-dependent manner up to 10 ng/mL, while effects of 20 ng/mL were approximately equal to those of 10 ng/mL. HGF stimulated uPA production beyond that in control cultures from 8 h until 48 h after HGF addition. HGF stimulated a uPA/uPAR proteolytic network in DU145 cells, which may be important for acquisition invasive potential by prostate cancer.

References

May 1, 1992·Clinical & Experimental Metastasis·M J Duffy
Jan 1, 1988·Annual Review of Cell Biology·O Saksela, D B Rifkin
Aug 16, 1984·Biochemical and Biophysical Research Communications·T NakamuraA Ichihara
Jan 1, 1982·Annals of the New York Academy of Sciences·K Ramasharma, M R Sairam
Feb 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Blasi
Apr 1, 1996·Journal of Biochemistry·K Matsumoto, T Nakamura
Sep 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M JeffersG F Vande Woude
Feb 10, 1998·International Journal of Cancer. Journal International Du Cancer·C FestucciaM Bologna
Jun 13, 1998·International Journal of Urology : Official Journal of the Japanese Urological Association·K NishimuraA Okuyama
Jan 1, 1999·Clinical & Experimental Metastasis·C FestucciaM Bologna

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Citations

Feb 23, 2012·The Protein Journal·Melissa Hirsch KuchmaPhilip A Arlen
Feb 3, 2005·Journal of Gastroenterology and Hepatology·Yoshihito ImaiKazuichi Okazaki
Feb 19, 2010·Folia Histochemica Et Cytobiologica·Ewa LukasiewiczMarcin Majka
Oct 16, 2012·Pharmacology & Therapeutics·Jing Zhang, Jinsong Liu
Jun 22, 2012·Journal of Cellular Physiology·Balakrishnan Chakrapani NarmadaHanry Yu
Aug 26, 2004·Advances in Cancer Research·Beatrice S Knudsen, Magnus Edlund
Aug 19, 2007·The Journal of Urology·Shahrokh F ShariatPierre I Karakiewicz
Dec 6, 2017·Journal of Theoretical Biology·Anna Konstorum, John S Lowengrub

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