Effects of hepatocyte growth factor on phosphorylation of extracellular signal-regulated kinase and hippocampal cell death in rats with transient forebrain ischemia

European Journal of Pharmacology
Makiko NiimuraSatoshi Takeo

Abstract

Hepatocyte growth factor (HGF) has been implicated in protection against several types of cell injuries. We investigated the effects of human recombinant HGF (hrHGF) on the selective neuronal cell death in the hippocampal CA1 region after transient forebrain ischemia in rats and explored the nature of the intracellular signaling pathway for the protection against this neuronal injury. hrHGF was injected continuously into the hippocampal CA1 region directly using an osmotic pump from 10 min to 72 h after the start of reperfusion. The marked increase in the number of TUNEL-positive cells found in the CA1 region after ischemia was almost completely abolished by the hrHGF treatment. Akt phosphorylation as well as IkappaB phosphorylation, which has been implicated in events downstream of the Akt, was not affected by hrHGF treatment. Extracellular signal-regulated kinase (ERK) phosphorylation was decreased in the CA1 region with time after ischemia. hrHGF increased or recovered ERK phosphorylation without changing the total amount of ERK protein. Immunohistochemical analysis demonstrated that phosphorylated ERK was colocalized with a neuronal nucleus marker NeuN in the hippocampal CA1 region of ischemic rats with hrHGF treatment at t...Continue Reading

References

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Citations

Sep 8, 2011·The European Journal of Neuroscience·Charles Finsterwald, Jean-Luc Martin
May 8, 2013·Molecular and Cellular Endocrinology·Smita Salian-MehtaMargaret E Wierman
Feb 13, 2015·Frontiers in Cellular Neuroscience·Phillip M UribeAllison B Coffin
Dec 2, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Thorsten R DoeppnerMathias Bähr
Jan 15, 2008·Journal of Neuroscience Research·Nik SaweHeng Zhao
Mar 3, 2019·International Journal of Molecular Sciences·Kazuya KitamuraMasaya Nakamura
Jun 29, 2021·Frontiers in Cell and Developmental Biology·Claudia DesoleTiziana Crepaldi

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