Effects of hepatocyte growth factor, transforming growth factor-beta1 and epidermal growth factor on bovine corneal epithelial cells under epithelial-keratocyte interaction in reconstruction culture

Experimental Eye Research
T NishimuraH Sugihara

Abstract

In the cornea, corneal epithelial cells are in close contact with keratocytes: the epithelial cells organize thickened lamellar structure on a layer of keratocytes embedded in extracellular matrix (ECM). Thus, growth factors are expected to critically regulate corneal component cells under epithelial-keratocyte interaction. The purpose of this study is to clarify effects of hepatocyte growth factor (HGF), transforming growth factor-beta1 (TGF-beta1) or epidermal growth factor (EGF) on corneal epithelial cells under epithelial-keratocyte interaction. We examined proliferation and differentiation of the epithelial cells in a simple corneal reconstruction culture composed of an epithelial cell layer on the keratocyte-containing stromal layer, using three-dimensional collagen gel matrix culture. We observed the morphological change by phase contrast microscopy, and conducted histological and immunohistochemical examinations. The epithelial proliferation was examined by nuclear bromodeoxy-uridine (BrdU) uptake. In the reconstructed cornea under epithelial-keratocyte interaction, EGF-, TGF-beta1- and HGF-treated cells formed a thickened epithelial layer consisting of 5-6, 5-6 and 3-4 cells, respectively. In fact, both EGF and TGF-bet...Continue Reading

References

Jan 1, 1992·Acta Ophthalmologica. Supplement·G SchultzS MacKay
May 1, 1992·Cell·B M Gumbiner
Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H SugiharaY Minami
Nov 1, 1990·Cell Regulation·M B Sporn, A B Roberts
Oct 23, 1986·Journal of Immunological Methods·N J GonchoroffM R Loken
Jan 1, 1985·Experimental Eye Research·P G WoostG S Schultz
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Sep 18, 1973·Biochemical and Biophysical Research Communications·L PickartM M Thaler
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Jul 1, 1983·The Journal of Investigative Dermatology·M PruniérasD Woodley
Apr 1, 1984·Current Eye Research·G PetroutsosY Courtois
Dec 1, 1993·Current Opinion in Cell Biology·E D Hay
Jun 1, 1996·Archives of Otolaryngology--head & Neck Surgery·T YamaguchiH Sugihara

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Citations

Sep 26, 2006·Tissue Engineering·Ken KobayashiKoichi Omori
May 1, 2007·Regenerative Medicine·Sajjad AhmadMajlinda Lako
Jun 13, 2002·Acta Ophthalmologica Scandinavica·Heather C Baldwin, John Marshall
Sep 26, 2015·The British Journal of Ophthalmology·Leopoldo SpadeaPaolo Trabucco
Jul 4, 2013·Acta Ophthalmologica·Nick J R Maycock, John Marshall
Jun 14, 2002·Biochemical and Biophysical Research Communications·Shuji TodaHajime Sugihara
Mar 16, 2005·Journal of Cataract and Refractive Surgery·Louise M Carrington, Mike Boulton
Feb 27, 2004·Biochemical and Biophysical Research Communications·Koichi UnoKenji Oshima
Aug 26, 2004·Advances in Cancer Research·Beatrice S Knudsen, Magnus Edlund
Nov 15, 2011·Colloids and Surfaces. B, Biointerfaces·Tsung-Jen WangTai-Horng Young
Aug 16, 2015·The Annals of Otology, Rhinology, and Laryngology·Yuta NakaegawaKoichi Omori
Jan 1, 2012·Journal of Functional Biomaterials·Samantha L WilsonYing Yang
Nov 5, 2014·Biomedicines·Ognoon MungunsukhRegina M Day
Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D GoukassianV Andrés
Dec 31, 2014·Biomedicines·Gianluca Baldanzi, Andrea Graziani
Jan 7, 2015·Molecular and Cellular Biochemistry·Prosun DasSujata Law
Jul 11, 2001·Experimental Biology and Medicine·L LuW W Kao
Jul 20, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Kosuke KawataShuji Toda

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