PMID: 9639739Aug 1, 1997Paper

Effects of herpes simplex virus thymidine kinase gene transduction and prodrug on ovarian cancer cell

Zhonghua fu chan ke za zhi
Z LiangZ Lin

Abstract

To evaluate the efficacy of gene therapy with the herpes simplex virus thymidine kinase (HSV-TK) gene transference followed by ganciclovir (GCV) therapy in ovarian cancer. HSV-TK gene was inserted into the hind III site of pLNSX vector and recombinant plasmid (pLNS/HSV-TK) was constracted. Then, the recombinant plasmid was transfered into PA317 packaged cell and the producer cell line of recombinant retrovirus vector pLNS/HSV-TK was established. The cytotoxicity efficacy of HSV-TK/GCV system to AO cell line was evaluated by microcucture tetrajolium test (MTT) method. The recombinant retroviral vector pLNS/HSK-TK can transfer HSV-SK gene into the genome of AO cell line and make it sensitive to GCV. The growth inhibitory rate of AO cell line transfered by pLNS/HSV-TK was 98.0% in the medium containing 400 mumol GVC. The ovarian cancer cell line transfered by HSV-TK gene regressed rapidly in response to the GCV therapy.

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.