Effects of Hg2+ and CH3Hg+ on Ca2+ fluxes in rat brain microsomes

Brain Research
A J FreitasD O Souza

Abstract

A permanent increase in cytosolic Ca2+ levels seems to be associated with various pathological situations which may result in cell death. Hg2+ and CH3Hg+ are potent neurotoxic agents, but the precise molecular mechanism(s) underlying their effects are not sufficiently understood. In the present study we investigated the potential role of Ca(2+)-ATPase located in the endoplasmic reticulum as a molecular target for mercury. Hg2+ and CH3Hg+ inhibited Ca(2+)-ATPase and Ca2+ uptake by brain microsomes with similar potencies. However, the inhibitory potency of Hg2+ was higher than that of CH3Hg+, probably reflecting differences in the affinity for the sulfhydryl groups of these compounds. Passive or unidirectional Ca2+ efflux (measured in the absences of Ca(2+)-ATPase ligands) was increased significantly by CH3Hg+ and Hg2+. Again, the potency of Hg2+ was higher than that of CH3Hg+. Blockers of Ca2+ channels (ruthenium red, procaine, heparin) did not affect the increase in passive Ca2+ efflux induced by mercury compounds, possibly indicating that Ca2+ release occurs through Ca(2+)-ATPase. Addition of physiological concentrations of glutathione (GSH) simultaneously with mercury abolished the inhibitory effects of both forms of Hg on ca...Continue Reading

References

Jun 25, 1975·Biochimica Et Biophysica Acta·E E Trotta, L de Meis
May 11, 1992·Annals of the New York Academy of Sciences·P Nicotera, S Orrenius
Jun 1, 1991·Pharmacology & Toxicology·A RossiP Nicotera
Dec 6, 1991·Brain Research·J Shah, H C Pant
Jun 1, 1988·Archives of Biochemistry and Biophysics·J J AbramsonG Salama
Apr 21, 1987·Brain Research·A SlivkaG Cohen
Jan 1, 1972·Annual Review of Pharmacology·T W Clarkson
Nov 1, 1980·Journal of Neurochemistry·A J CooperT E Duffy
Oct 1, 1993·Toxicology and Applied Pharmacology·M F DennyW D Atchison
Feb 15, 1995·The Biochemical Journal·J C BenechL de Meis
Nov 1, 1994·Neuroscience·P Kostyuk, A Verkhratsky
Mar 1, 1994·Journal of Neurochemistry·S Eimerl, M Schramm
Dec 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A D RossiP Nicotera
Jan 1, 1993·Annual Review of Pharmacology and Toxicology·T W Clarkson
Dec 1, 1995·Toxicology and Applied Pharmacology·M F Hare, W D Atchison

❮ Previous
Next ❯

Citations

Jul 2, 2003·Neurotoxicity Research·Coral SanfeliuEduard Rodríguez-Farré
Jan 11, 2005·Neurotoxicity Research·Juan Segura Aguilar, Richard M Kostrzewa
Oct 15, 2013·BMC Genomics·Matthew K McElweeJonathan H Freedman
May 24, 2011·Toxicology and Applied Pharmacology·Marcelo FarinaJoão B T Rocha
Jan 24, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Daniel H RoosJoão B T Rocha
Feb 3, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·B BurlandoA Viarengo
May 14, 2004·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Maria Beatriz MorettoMaría Rosa Chitolina Schetinger
Dec 26, 2012·Neurochemistry International·Marcelo FarinaMichael Aschner
Feb 27, 2001·Ecotoxicology and Environmental Safety·L R FaroR Durán
Jul 27, 2007·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Rainald MössnerPeter Riederer
Jun 20, 2003·Journal of Biochemical and Molecular Toxicology·M B MorettoJ B T Rocha
Sep 23, 2014·The Journal of Toxicological Sciences·Hironori KandaYoshito Kumagai
Jul 19, 2020·Environmental Health and Preventive Medicine·Jin-Yong LeeMasahiko Satoh
Mar 27, 2003·Neuroreport·Lisiane O PorciúnculaDiogo O Souza
Mar 1, 2008·Neurochemical Research·Anna M A P FernandesRoger F Castilho
Sep 17, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ilya O KurochkinInna M Sokolova
Dec 19, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Zuzana KnazickaPeter Massanyi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.