Abstract
Hg2+ is an environmental pollutant that adversely affects a range of cellular functions, including those that regulate free cytosolic Ca2+ (Ca(i)2+). To investigate the mechanism of Hg(2+)-induced Ca(i)2+ signaling, we examined the effects of Hg2+ on Ca(i)2+ in isolated skate hepatocytes, and developed a method to assess cytosolic Hg2+ (Hgi2+) in these cells as well. At lower concentrations (1-5 microM), Hg2+ induced little detectable change in Ca(i)2+. At higher concentrations (10 microM-1 mM), Hg2+ induced a dose-dependent, progressive increase in Ca(i)2+, which occurred even in Ca(2+)-free medium. Pretreatment of hepatocytes with the membrane-impermeant Hg2+ chelator glutathione (GSH) blocked the Hg(2+)-induced Ca(i)2+ increase, whereas addition of GSH after exposure to Hg2+ slowed but did not prevent further increases in Ca(i)2+. Pretreatment with the membrane-permeant Hg2+ chelator dithiothreitol (DTT) also blocked Hg(2+)-induced increases in Ca(i)2+. Unlike GSH, however, addition of DTT after Hg2+ significantly decreased Ca(i)2+, returning it to near-baseline levels. Thapsigargin induced a sustained increase in Ca(i)2+, but subsequent addition of Hg2+ resulted in a further, progressive Ca(i)2+ increase. We also describe t...Continue Reading
References
Jan 1, 1978·International Review of Cytology·N S Kosower, E M Kosower
Jul 23, 1992·Nature·R RizzutoT Pozzan
Aug 1, 1992·The Journal of Physiology·G SalamaG K Pike
Jan 1, 1992·Molecular Biology of the Cell·M H Nathanson, A D Burgstahler
Dec 1, 1991·Environmental Health Perspectives·W F Fitzgerald, T W Clarkson
Sep 1, 1991·Toxicology Letters·S C Bondy, M McKee
Feb 1, 1991·Toxicology and Applied Pharmacology·M SellingerJ L Boyer
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M W SmithB F Trump
Jun 15, 1990·The Biochemical Journal·V Shoshan-BarmatzN Kraus-Friedmann
Dec 14, 1990·Biochemical and Biophysical Research Communications·K Y Jung, H Endou
Sep 15, 1988·Toxicology and Applied Pharmacology·N BallatoriJ L Boyer
May 15, 1988·The Biochemical Journal·A EnyediJ T Penniston
Jun 1, 1985·Ecotoxicology and Environmental Safety·S K BansalS V Chandra
Nov 12, 1987·Biochimica Et Biophysica Acta·M W SmithB F Trump
Apr 18, 1985·Nature·A P SomlyoA V Somlyo
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J J AbramsonG Salama
Aug 1, 1982·The Journal of Cell Biology·R Y TsienT J Rink
Dec 1, 1993·Pflügers Archiv : European journal of physiology·A KoivistoJ Nedergaard
Jun 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W D Atchison, M F Hare
Jan 1, 1995·Archives of Toxicology·F WeinsbergH Wiegand
Jul 11, 1994·FEBS Letters·F IchasE L Holmuhamedov
Sep 1, 1994·The Journal of Cell Biology·R RizzutoT Pozzan
Apr 2, 1993·Brain Research·J AlbrechtM Aschner
Dec 31, 1993·Brain Research·M PekelD Büsselberg
Dec 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A D RossiP Nicotera
Feb 1, 1993·Journal of Toxicology and Environmental Health·X X TanL G Costa
Citations
Dec 6, 2005·Cell Biology and Toxicology·I AduayomC Jumarie
Mar 24, 2004·Aquatic Toxicology·B MarchiA Viarengo
Jul 31, 2003·Cell Calcium·Bruno BurlandoAldo Viarengo
Nov 5, 2002·Aquatic Toxicology·Elena FabbriAntonio Capuzzo
May 5, 1998·Journal of Pharmacological and Toxicological Methods·J W Kleineke, I A Brand
Nov 16, 2013·Environmental Science and Pollution Research International·Milena HorvatJadran Faganeli
Apr 14, 2012·Journal of Fish Biology·D W Barnes
Feb 3, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·B BurlandoA Viarengo
Jan 27, 2005·Cell Calcium·Ana-Maria FloreaDietrich Büsselberg
Oct 18, 2000·Cell Calcium·B MarchiA Viarengo
Jun 16, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Barbara MarchiGabriella Gallo
Jan 25, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Martha A RatnerJohn N Forrest
Jan 30, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martine AvellaPhilippe Poujeol
Jul 13, 2002·The Journal of Experimental Zoology·Marie Goudeau, Henri Goudeau
Jun 13, 1998·Biochimica Et Biophysica Acta·F C Mooren, R K Kinne