Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts

Scientific Reports
Zongliang JiangXiuchun Cindy Tian

Abstract

High hydrostatic pressure (HHP) has been used to pre-condition embryos before essential, yet potentially detrimental procedures such as cryopreservation. However, the mechanisms for HHP are poorly understood. We treated bovine blastocysts with three different HHP (40, 60 and 80 MPa) in combination with three recovery periods (0, 1 h, 2 h post HHP). Re-expansion rates were significantly higher at 40 and 60 but lower at 80 MPa after vitrification-warming in the treated groups than controls. Microarray analysis revealed 399 differentially expressed transcripts, representing 254 unique genes, among different groups. Gene ontology analysis indicated that HHP at 40 and 60 MPa promoted embryo competence through down-regulation of genes in cell death and apoptosis, and up-regulation of genes in RNA processing, cellular growth and proliferation. In contrast, 80 MPa up-regulated genes in apoptosis, and down-regulated protein folding and cell cycle-related genes. Moreover, gene expression was also influenced by the length of the recovery time after HHP. The significantly over-represented categories were apoptosis and cell death in the 1 h group, and protein folding, response to unfolded protein and cell cycle in the 2 h group compared to ...Continue Reading

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Citations

Mar 16, 2017·Reproduction in Domestic Animals = Zuchthygiene·N WangX-M Zhao
Feb 7, 2017·PloS One·Bernadett FaragóJudit Cserepes
Oct 6, 2017·Biopreservation and Biobanking·Guobo QuanQionghua Hong
Dec 14, 2019·Current Stem Cell Research & Therapy·Oznur BaskanEngin Ozcivici
May 9, 2021·Cryobiology·José Carlos Ferreira-SilvaMarcos Antonio Lemos Oliveira
Jul 15, 2021·Biology Open·Kazuko OkamotoHideaki Fujita

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Datasets Mentioned

BETA
GSE7559

Methods Mentioned

BETA
protein folding
RNA-seq
light microscopy

Software Mentioned

7500
GenePix Pro
DAVID
R
Genespring
Genespring GX

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