Effects of high-intensity interval exercise on muscle fatigue and SR function in rats: a comparison with moderate-intensity continuous exercise.

Journal of Applied Physiology
Chihiro AibaraMasanobu Wada

Abstract

In this study, we compared muscle fatigue induced by high-intensity interval exercise (HIIE) and moderate-intensity continuous exercise (MICE), with a focus on changes in the function of sarcoplasmic reticulum (SR) and myofibril. To achieve the aim of this study with mechanically skinned fibers with sealed transverse tubules and intact SR membrane, myofibrillar Ca2+ sensitivity, depolarization-induced force, and action potential-induced force were evaluated. Rat gastrocnemius muscles were subjected to HIIE-mimicking or MICE-mimicking stimulation in situ. The number of contractions was the same for MICE- and HIIE-mimicking stimulation (total of 360 contractions). Three hours after cessation of stimulation, the superficial regions of gastrocnemius muscles were dissected and used for biochemical and skinned fiber analyses. At 3 h of recovery, forces at 20 and 100 Hz in whole muscles had returned to resting levels in MICE but not HIIE muscles. The reduced glutathione content was decreased only in HIIE muscles. Both MICE- and HIIE-mimicking stimulation resulted in an increase in myofibrillar Ca2+ sensitivity in skinned fibers. Only HIIE-mimicking stimulation led to a decrease in the ratio of force at 1 Hz to that at 50 Hz and the ra...Continue Reading

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Citations

Feb 18, 2021·American Journal of Physiology. Cell Physiology·Daiki Watanabe, Masanobu Wada

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