Effects of high-pressure processing on Toxoplasma gondii tissue cysts in ground pork

The Journal of Parasitology
David S LindsayJ P Dubey

Abstract

Ingestion of Toxoplasma gondii tissue cysts can result in severe disease in immunocompromised individuals and pregnant women. Treatment of meat and meat products to eliminate viable T. gondii tissue cysts would provide a means to protect consumers. In this study, we examined the effects of high-pressure processing (HPP) on ground pork containing viable tissue cysts of the VEG strain of T. gondii. Ground pork containing tissue cysts was exposed to 400, 300, 200, 100, or 0 MPa treatment for 30, 60, or 90 sec in a commercial HPP unit. The HPP-treated ground pork was subjected to acid-pepsin digestion and bioassayed in mice. The results of the mouse bioassay revealed that none of the mice inoculated with tissue cysts exposed to 400 or 300 MPa became infected, whereas all mice inoculated with tissue cysts exposed to 200, 100, or 0 MPa became infected with T. gondii regardless of exposure time. Results indicate that HPP treatment of ground pork with 300 MPa of pressure will render tissue cysts of T. gondii nonviable and make pork safe for human consumption.

References

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Aug 23, 2005·The Journal of Parasitology·David S LindsayJ P Dubey
Apr 25, 2006·The Journal of Parasitology·Angela D CoullietteJoan B Rose

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Citations

Apr 12, 2012·Clinical Microbiology Reviews·Florence Robert-Gangneux, Marie-Laure Dardé
Jul 9, 2008·The Journal of Parasitology·David S LindsayJ P Dubey
Mar 2, 2016·Parasitology·V DjokicP Boireau
Jul 8, 2010·Meat Science·G H ZhouY Liu
Jan 1, 2008·Meat Science·T AymerichJ M Monfort
Oct 31, 2006·The Veterinary Clinics of North America. Food Animal Practice·J P Dubey, David S Lindsay
Aug 13, 2008·International Journal for Parasitology·Aize Kijlstra, Erik Jongert
Feb 14, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·O Djurković-DjakovićJ Dupouy-Camet
Dec 5, 2006·Preventive Veterinary Medicine·Jean Carlos Ramos SilvaJosé Soares Ferreira Neto
Jul 11, 2006·Veterinary Parasitology·S Solaymani-Mohammadi, W A Petri
May 12, 2009·Memórias do Instituto Oswaldo Cruz·Astrid M Tenter
Oct 23, 2018·Pathogens and Global Health·Adel Mirza AlizadehHedayat Hosseini
Dec 4, 2018·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Lucy Robertson
Aug 18, 2009·Journal of Food Protection·Adrienne E H Shearer, Kalmia E Kniel
Aug 2, 2017·Foodborne Pathogens and Disease·Alberto De BerardinisAlberto Vergara
Jun 27, 2013·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)
Jun 1, 2021·Comprehensive Reviews in Food Science and Food Safety·Kemal AganovicVolker Heinz

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Methods Mentioned

BETA
light microscopy

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