PMID: 8611662Apr 12, 1996Paper

Effects of HMG-CoA reductase inhibitors on growth and differentiation of cultured rat skeletal muscle cells

Biochimica Et Biophysica Acta
J H VeerkampA Oosterhof

Abstract

HMG-CoA reductase inhibitors have been associated with skeletal muscle myopathy, ranging from asymptomatic elevations of serum creatine kinase (CK) activity to rhabdomyolysis. In this study, we assessed the effects of addition of different concentrations of simvastatin and pravastatin on growth and differentiation of cultured primary rat skeletal muscle cells. Protein concentrations, CK activity and percentage CK-MM, which is a parameter for maturation, were determined. Effects were generally stronger if inhibitors were added to both growth and differentiation medium rather than only to differentiation medium. Addition of 25 microM pravastatin caused only a decrease of CK activity. Addition of 1-5 microM simvastatin resulted in a decrease of protein concentration, CK activity and percentage CK-MM, whereas 25 microM simvastatin resulted in cell death. Addition of mevalonic acid or cholesterol could not prevent the effects of 1 microM simvastatin. In addition, 1 microM simvastatin did not influence the cholesterol and phospholipid content of the cells. Superfusion of cultured cells with simvastatin concentrations of 10 microM and higher caused a transient increase of the cytoplasmic calcium concentration followed by an apparent s...Continue Reading

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Citations

May 26, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·T KitaoH Ishizu
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·S RaoK Keyomarsi
Sep 25, 2004·Muscle & Nerve·Olavo M Vasconcelos, William W Campbell
Mar 20, 1999·The Annals of Pharmacotherapy·J C Lin, M K Ito
Jun 29, 2018·Oxidative Medicine and Cellular Longevity·Anna JaśkiewiczArkadiusz Orzechowski

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