Effects of HMGB1 on proliferation and apoptosis of human brain glioma CD133 cells

Bratislavské lekárske listy
W P Zhao, Q X Chen

Abstract

To understand the effects of HMGB1 recombinant lentivirus vector on proliferation and apoptosis on CD133 cells after transfecting a human glioma cell strain CD133. The CD133 human glioma cell strain cultured in vitro was transfected with HMGB1 lentiviral vector and empty vector, respectively, while CD133 cells cultured normally were used as the blank control. Changes in HMGB1 protein level were detected with western blot hybridization; apoptosis was detected with a flow cytometer; the cell proliferation was analysed with MTT method. The results from the western blot hybridization indicated that the expression level of HMGB1 protein in the group infected with HMGB1 recombinant over-expressed lentiviral vector increased significantly (p<0.05) compared with the blank control and CD133 cells transfected with the empty vector. The absorbance value of cell proliferation in the group infected with HMGB1 over-expressed lentiviral vector increased gradually on days 1, 2, 3, 4 and 5, but the difference was significant on day 2 after infection and later compared with the blank control group and negative control group (p<0.05). Based on detection with a flow cytometer, the apoptosis rate of CD133 cells transfected with HMGB1 over-expressed...Continue Reading

Citations

Jun 6, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Efthalia AngelopoulouAthanasios G Papavassiliou

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